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Con A affinity glycoproteomics of normal human liver tissue

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Abstract

In order to establish the novel high throughput, high efficiency and low cost technological platform for the research of N-glycoproteomics, to resolve the significance of characteristic expression profile of glycoprotein and to find the proteins with biological functional importance, the glycoproteins with high-mannose core and the two antennary types were purified and enriched by the Con A affinity chromatography. Con A affinity protein expression profiles of normal human liver tissue were generated by using SDS-PAGE, two-dimensional electrophoresis (2-DE) followed by fast fluorescence staining based on multiplexed proteomics (MP) technology. 301 visible protein spots on the gel were detected and 85 of glycoproteins were further successfully identified via peptide mass fingerprinting (PMF) by a matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS/MS) and annotated to IPI databases. Identified glycoproteins definitely take part in the regulation of cell cycle and metabolic processes. The glycosylation sites were predicted with NetNGlyc 1.0 and NetOGlyc 3.1 software, meanwhile they were classified according to the geneontology methods. The construction of Con A affinity glycoprotein database of normal human liver tissue would contribute to the subsequent research.

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Authors and Affiliations

  1. Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, 200032, China

    Sun QiangLing, Liu YinKun, Lu WenJing, Zhou HaiJun, Dai Zhi & Guo Kun

  2. System Biology Lab, Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, China

    Cheng Gang

  3. Research Center for Proteomics, Institute of Biomedical Sciences, Fudan University, Shanghai, 200433, China

    Zhou XinWen, Wei LiMing & Lu HaoJie

Authors
  1. Sun QiangLing
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  2. Liu YinKun
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  3. Lu WenJing
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  4. Cheng Gang
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  5. Zhou HaiJun
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  6. Zhou XinWen
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  7. Wei LiMing
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  8. Dai Zhi
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  9. Guo Kun
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  10. Lu HaoJie
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Corresponding author

Correspondence to Liu YinKun.

Additional information

Supported by the National Basic Research Program of China (973)(Grant No. 2004CB520802)

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Sun, Q., Liu, Y., Lu, W. et al. Con A affinity glycoproteomics of normal human liver tissue. SCI CHINA SER C 50, 403–411 (2007). https://doi.org/10.1007/s11427-007-0027-z

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  • DOI: https://doi.org/10.1007/s11427-007-0027-z

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