Abstract
This paper investigated the correlation between polymorphisms and haplotypes in the apolipoprotein B (apoB) gene (SP-I/D, Xbal-RFLP, VNTR) and natural longevity persons among the Uygur people in Xinjiang. For this purpose, 191 healthy Uygur individuals aged above 90 from Hetian area of Xinjiang were recruited, and another 53 persons aged 65–70 from the same nationality, the same region and with the same gender ratio, served as the control group. Genotyping was performed by PCR-SSP, PCR-RFLP and PCR-sequencing methods. Logistic regression analyses revealed that the frequencies of X+X+ genotype, M and L alleles and the genetypes composed of M and L were significantly higher in the longevity group than in the control group. In haplotype analyses, we found that, in the long-lived people, the frequency of haplotypes composed of the X+ and M alleles was significantly higher whereas the frequency of haplotypes composed of the X-and S alleles was significantly lower (both P<0.05) I than those of their controls. These results indicated that the S allele, SS genotype and X+-S, D-S, D-X+-S haplotypes were the possible adverse factors, whereas the M, L alleles, X+X+, MM, ML, LL genotypes and I-X+-M, X+-M haplotypes were the possibe protective factors for the naturally long-lived Uygur people in China.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Atzmon G, Rincon M, Rabizadeh P, et al. Biological evidence for inheritance of exceptional longevity. Mech Ageing Dev, 2005, 126(2): 341–345
De Benedictis G, Falcone E, Rose G, et al. DNA multiallelic systems reveal gene/longevity associations not detected by diallelic systems. The APOB locus. Hum Genet, 1997, 99: 312–318
Arias-Vasquez A, Sayed-Tabatabaei F A, Schut A F, et al. Angiotensin converting enzyme gene, smoking and mortality in a population-based study. Eur J Clin Invest, 2005, 35(7): 444–449
van Heemst D, Moojiaart S P, Beekman M, et al. Variation in the human TP53 gene affects old age survival and cancer mortality. Exp Gerontol, 2005, 40(1–2): 11–15
Franceschi C, Olivieri F, Marchegiani F, et al. Genes involved in immune response/inflammation, IGF1/insulin pathway and response to oxidative stress play a major role in the genetics of human longevity: The lesson of centenarians. Mech Ageing Dev, 2005, 126(2): 351–361
Lusis A J, West R, Mehrabian M, et al. Cloning and expression of apolipoprotein B, The major protein of low and very low density lipoproteins. Proc Natl Acad Sci USA, 1985, 82: 4597–4601
Knott T J, Pease R J, Powell L M, et al. Complete protein sequence and identification of structural domains of human apolipoprotein B. Nature, 1986, 323: 734–738
Masana L I, Febrer G, Cavanna J, et al. Common genetic variants that release to disorders of lipid transport in Spanish subjects with premature coronary artery disease. Clin Sci, 2001, 100: 183–190
Benes P, Muzik J, Benedı’k J, et al. Single effects of apolipoproteinB, (a), and E polymorphisms and interaction between plasminogen activator inhibitor-1 and apolipoprotein(a) genotypes and the risk of coronary artery disease in Czech male Caucasians. Mol Genet Metab, 2000, 69: 137–143
de Padua Mansur A, Annichino-Bizzacchi J, Favarato D, et al. Angiotensin-converting enzyme and apolipoprotein B polymorphisms in coronary artery disease. Am J Cardiol, 2000, 85: 1089–1093
Singh M K, Pandey U B, Ghoshal U C, et al. Apolipoprotein B-100 Xba I gene polymorphism in gallbladder cancer. Hum Genet, 2004, 114: 280–283
Vital Statistics Governmental Department of State Statistics Bureau, ed. Annual Report Forms of China Vital Statistics. Beijing: China Statistics Publishing Company, 1993. 52
De Benedictis G L, Carotenuto G, Carrier M, et al. Age-related changes of the 3’APOB-VNTR genotype pool in ageing cohorts. Ann Hum Genet, 1998, 62: 115–122
Boerwinkle E, Xiong W, Fourest E, et al. Rapid typing of tandemly repeated hypervariable loci by the polymerase chain reaction: Application to the apolipoprotein B 3-prime hypervariable region. Proc Natl Acad Sci USA, 1989, 86: 212–216
Bernard S, Charriere S, Charcosset M, et al. Relation between XbA1 apolipoprotein B gene polymorphism and cardiovascular risk in a type 2 diabetic cohort. Atherosclerosis, 2004, 175(1): 177–181
Puri R D, Tewari S, Sinha N, et al. Polymorphisms in the apolipoprotein B-100 gene: association with plasma lipid concentration and coronary artery disease. Indian Heart J, 2003, 55(1): 60–64
de Padua Mansur A, Annichino-Bizzacchi J, Favarato D, et al. Angiotensin-converting enzyme and apolipoprotein B polymorphisms in coronary artery disease. Am J Cardiol, 2000, 85: 1089–1093
Garaso S, Berardelli M, DeRango F, et al. A study of the average effect of the 3’APOB-VNTR poltmorphism on lipidemic parameters could explain why the short alleles(<35 repeats) are rare in centenarians. BMC Med Genet, 2004, 9(5): 3.
Friedl W, Ludwig E H, Paulweber B, et al. Hypervariability in a minisatellite 3’ of the apolipoprotein B gene in patients with coronary heart disease compared with normal controls. J Lipid Res, 1990, 31(4): 659–665
Ye P, Chen B, Wang S. Association of polymorphisms of the apolipoprotein B gene with coronary heart disease in Han Chinese. Atherosclerosis, 1995,117: 43–50
Heng C K, Saha N, Low P S. Evolution of the apolipoportein B gene and coronary artery disease: A study in low and high risk Asians. Ann Hum Genet, 1999, 63: 45–62
Benes P, Muzik J, Benedı’k J, et al. Apolipoprotein B signal peptide polymorphism in relation to lipids and diabetes in male CAD patients. Atherosclerosis, 2000, 152: 257–258
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Jiang, W., Qiu, C., Cheng, Z. et al. Correlation between haplotype of apolipoprotein B gene and natural longevity persons in Uygur Nationality. SCI CHINA SER C 50, 80–87 (2007). https://doi.org/10.1007/s11427-007-0008-2
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11427-007-0008-2