Abstract
OsPT6:1, a phosphate transporter encoding gene from the leaf samples of Oryza sativa, was identified through PCR with specifically designed primers. The phylogenetic analysis and the conserved amino acid residue site detection suggested OsPT6:1 a possible high-affinity phosphate transporter encoding gene. In situ hybridization and RT-PCR demonstrated the expression of OsPT6:1 in both roots and leaves. The peak expression signal was observed in mesophyll cells under low phosphorus (P) induction. A homologous recombination study indicated that OsPT6:1 can enhance the Pi uptake efficiency of Pichia pastoris. At the meantime, the introduction of OsPT6:1 was able to complement the Pi uptake function of yeast cells with high-affinity phosphate transporters deficient. Those results substantiated our contention that OsPT6:1 encoded a high-affinity phosphate transporter of Oryza sativa.
Similar content being viewed by others
References
Sanchez P A, Uehara G. Management consideration for acid soils with high phosphorus fixation capacity. In: Khasawneh F E, Sample E C, Kamprath E J, eds. The Role of Phosphorus in Agriculture. American Society of Agronomy, Madison, Wisconsin, USA, 1980. 417–514
Barber S A, Walker J M, Vasey E H. Mechanisms for the movement of plant nutrients from the soil and fertilizer to the plant root. J Agric Food Chem, 1963, 11: 204–207
Ullrich-Eberius C I, Novacky A, van Bel A J E. Phosphate uptake in Lenma gibba G1: energetics and kinetics. Planta, 1984, 161: 46–52
Marschner H. Mineral Nutrition in Higher Plants. San Diego: Academic Press, 1995
Zhao J, Fu J B, Liao H, He Y, Hu Y M, Qiu L J, et al. Characterization of root architecture in an applied core collection for phosphorus efficiency of soybean germplasm. Chin Sci Bull, 2004, 49(15): 1611–1620
Raghothama K G. Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol, 1999, 50: 665–693
Schachtman D P, Reid R J, Ayling, S M. Phosphorus uptake by plants: from soil to cell. Plant Physiol, 1998, 116: 447–453
Mimura T. Regulation of phosphate transport and homeostasis in plant cells. Int Rev Cytol, 1999, 191: 149–200
Liu C M, Muchhal U S, Raghothama K G. Differential expression of TPS11, a phosphate starvation-induced gene in tomato. Plant Molecular Biology, 1997, 33: 867–874
Smith F W, Ealing P M, Dong B, Delhaize E. The cloning of two Arabidopsis genes belonging to a phosphate transporter family. Plant J, 1997, 11: 83–92
Leggewie G, Willmitzer L, Riesmeier J W. Two cDNAs from potato are able to complement a phosphate uptake-deficient yeast mutant: identification of phosphate transporters from higher plants. Plant Cell, 1997, 9: 383–392
Liu H, Trieu A T, Blaylock L A, Harrison M J. Cloning and characterization of two phosphate transporters from Medicago truncatula roots: regulation in response to phosphate and to colonization by arbuscular mycorrhizal (AM) fungi. Mol Plant Microbe Interact, 1998, 11: 14–22
Rae A L, Cybinski D H, Jarmey J M, Smith F W. Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family, Plant Mol Biol, 2003, 53: 27–36
Mudge S R, Rae A L, Diatloff E, Smith F W. Expression analysis suggests novel roles for members of the Pht1 family of phosphate transporters in Arabidopsis. Plant J, 2002, 31: 341–353
Kai M, Takazumi K, Adachi H, Wasaki J, Shinano T, Osaki M. Cloning and characterization of four phosphate transporter cDNAs in tobacco. Plant Science, 2002, 163: 837–846
Mitsukawa N, Okumura S, Shirano Y, Sato S, Kato T, Harashima S, Shibata D. Overexpression of an Arabidopsis thaliana high-affinity phosphate transporter gene in tobacco cultured cells enhances cell growth under phosphate-limited conditions. Proc Natl Acad Sci USA, 1997, 94: 7098–7102
Liu J Q, Deborah A, Samac B B, Deborah L A, Carroll P V. Signaling of phosphorus deficiency-induced gene expression in white lupin requires sugar and phloem transport. Plant J, 2005, 41: 257–268
Karthikeyan A S, Varadarajan D K, Mukatira U T, D’Urzo M P, Damsz B, Raghothama K G. Regulated expression of Arabidopsis phosphate transporters. Plant Physiol, 2002, 130: 221–233
Paszkowski U, Kroken S, Roux C, Briggs S P. Rice phosphate transporters include an evolutionarily divergent gene specifically activated in arbuscular mycorrhizal symbiosis. Proc Natl Acad Sci USA, 2002, 99: 13324–13329
Author information
Authors and Affiliations
Corresponding author
Additional information
These authors contributed equally to this work.
Rights and permissions
About this article
Cite this article
Ming, F., Lu, Q., Wang, W. et al. Cloning, expression and function of phosphate transporter encoded gene in Oryza sativa L.. SCI CHINA SER C 49, 409–413 (2006). https://doi.org/10.1007/s11427-006-2030-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11427-006-2030-1