Skip to main content
SpringerLink
Log in

Chiral coating-mediated interactions of bacteria with diverse biointerfaces

  • Articles
  • Published:
Science China Chemistry Aims and scope Submit manuscript

Abstract

Bacteria, as living agents, have been widely used for microbial therapy due to their inherent ability to colonize different in vivo microenvironments, particularly in the gut and tumor. The interaction with diverse biointerfaces plays a critical role in bacterial colonization, and thereby determine the ultimate efficacy of microbial therapy. Although surface modification of bacteria with exogenous functional motifs can vary their interaction with surroundings, the effects of surface chirality of modified bacteria on mucous adhesion, tumor cell binding, and bacterial competition remain unknown. Here, we describe surface chirality-dependent selective interactions of bacteria to mucins, tumor cells, and pathogens. By coating bacteria with cationic polyethyleneimine modified with different chiral amino acids through electrostatic interaction, we find that bacteria coated with a D-chiral surface structure exhibit greatly increased adhesion to both mucins and tumor cells compared with those of L- and DL-structures. In addition, by adjusting the chirality of the coating, wrapped probiotic bacteria can selectively resist pathogenic bacteria, showing great potential to enhance colonization and positively modulate the gut and tumor microbiota. This work discloses surface chirality-dependent interaction of bacteria with different biointerfaces, showing a potential to tune the colonization and therapeutic effect of living bacterial agents for disease treatment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Dekaboruah E, Suryavanshi MV, Chettri D, Verma AK. Arch Microbiol, 2020, 202: 2147–2167

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  2. Hou K, Wu ZX, Chen XY, Wang JQ, Zhang D, Xiao C, Zhu D, Koya JB, Wei L, Li J, Chen ZS. Sig Transduct Target Ther, 2022, 7: 135

    Article  Google Scholar 

  3. Buffie CG, Bucci V, Stein RR, McKenney PT, Ling L, Gobourne A, No D, Liu H, Kinnebrew M, Viale A, Littmann E, van den Brink MRM, Jenq RR, Taur Y, Sander C, Cross JR, Toussaint NC, Xavier JB, Pamer EG. Nature, 2015, 517: 205–208

    Article  PubMed  CAS  Google Scholar 

  4. Knip M, Siljander H. Nat Rev Endocrinol, 2016, 12: 154–167

    Article  PubMed  CAS  Google Scholar 

  5. Zheng DW, Dong X, Pan P, Chen KW, Fan JX, Cheng SX, Zhang XZ. Nat Biomed Eng, 2019, 3: 717–728

    Article  PubMed  CAS  Google Scholar 

  6. Nakatsuji T, Hata TR, Tong Y, Cheng JY, Shafiq F, Butcher AM, Salem SS, Brinton SL, Rudman Spergel AK, Johnson K, Jepson B, Calatroni A, David G, Ramirez-Gama M, Taylor P, Leung DYM, Gallo RL. Nat Med, 2021, 27: 700–709

    Article  PubMed  CAS  Google Scholar 

  7. Alexander JL, Wilson ID, Teare J, Marchesi JR, Nicholson JK, Kinross JM. Nat Rev Gastroenterol Hepatol, 2017, 14: 356–365

    Article  PubMed  CAS  Google Scholar 

  8. Eom T, Kim YS, Choi CH, Sadowsky MJ, Unno T. J Microbiol, 2018, 56: 189–198

    Article  PubMed  CAS  Google Scholar 

  9. Fu A, Yao B, Dong T, Chen Y, Yao J, Liu Y, Li H, Bai H, Liu X, Zhang Y, Wang C, Guo Y, Li N, Cai S. Cell, 2022, 185: 1356–1372.e26

    Article  PubMed  CAS  Google Scholar 

  10. Galeano Niño JL, Wu H, LaCourse KD, Kempchinsky AG, Baryiames A, Barber B, Futran N, Houlton J, Sather C, Sicinska E, Taylor A, Minot SS, Johnston CD, Bullman S. Nature, 2022, 611: 810–817

    Article  PubMed  PubMed Central  Google Scholar 

  11. Kalaora S, Nagler A, Nejman D, Alon M, Barbolin C, Barnea E, Ketelaars SLC, Cheng K, Vervier K, Shental N, Bussi Y, Rotkopf R, Levy R, Benedek G, Trabish S, Dadosh T, Levin-Zaidman S, Geller LT, Wang K, Greenberg P, Yagel G, Peri A, Fuks G, Bhardwaj N, Reuben A, Hermida L, Johnson SB, Galloway-Peña JR, Shropshire WC, Bernatchez C, Haymaker C, Arora R, Roitman L, Eilam R, Weinberger A, Lotan-Pompan M, Lotem M, Admon A, Levin Y, Lawley TD, Adams DJ, Levesque MP, Besser MJ, Schachter J, Golani O, Segal E, Geva-Zatorsky N, Ruppin E, Kvistborg P, Peterson SN, Wargo JA, Straussman R, Samuels Y. Nature, 2021, 592: 138–143

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  12. Chen Y, Du M, Yuan Z, Chen Z, Yan F. Nat Commun, 2022, 13: 4468

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  13. Houghteling PD, Walker WA. J Pediatr Gastroenterol Nutr, 2015, 60: 294–307

    Article  PubMed  PubMed Central  Google Scholar 

  14. Zheng D, Liwinski T, Elinav E. Cell Res, 2020, 30: 492–506

    Article  PubMed  PubMed Central  Google Scholar 

  15. Raman V, Van Dessel N, Hall CL, Wetherby VE, Whitney SA, Kolewe EL, Bloom SMK, Sharma A, Hardy JA, Bollen M, Van Eynde A, Forbes NS. Nat Commun, 2021, 12: 6116

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  16. Li SS, Zhu A, Benes V, Costea PI, Hercog R, Hildebrand F, Huerta-Cepas J, Nieuwdorp M, Salojärvi J, Voigt AY, Zeller G, Sunagawa S, de Vos WM, Bork P. Science, 2016, 352: 586–589

    Article  PubMed  CAS  Google Scholar 

  17. Ya’ari S, Halperin-Sternfeld M, Rosin B, Adler-Abramovich L. Int J Mol Sci, 2020, 21: 7370

    Article  PubMed  PubMed Central  Google Scholar 

  18. Wang L, Cao Z, Zhang M, Lin S, Liu J. Adv Mater, 2022, 34: 2106669

    Article  CAS  Google Scholar 

  19. Chen QW, Liu XH, Fan JX, Peng SY, Wang JW, Wang XN, Zhang C, Liu CJ, Zhang XZ. Adv Funct Mater, 2020, 30: 1909

    Google Scholar 

  20. Chen WH, Chen QW, Chen Q, Cui C, Duan S, Kang Y, Liu Y, Liu Y, Muhammad W, Shao S, Tang C, Wang J, Wang L, Xiong MH, Yin L, Zhang K, Zhang Z, Zhen X, Feng J, Gao C, Gu Z, He C, Ji J, Jiang X, Liu W, Liu Z, Peng H, Shen Y, Shi L, Sun X, Wang H, Wang J, Xiao H, Xu FJ, Zhong Z, Zhang XZ, Chen X. Sci China Chem, 2022, 65: 1010–1075

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  21. Luo H, Chen Y, Kuang X, Wang X, Yang F, Cao Z, Wang L, Lin S, Wu F, Liu J. Nat Commun, 2022, 13: 7808

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  22. Cao Z, Cheng S, Wang X, Pang Y, Liu J. Nat Commun, 2019, 10: 3452

    Article  PubMed  PubMed Central  Google Scholar 

  23. Cao Z, Wang X, Pang Y, Cheng S, Liu J. Nat Commun, 2019, 10: 5783

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  24. Kwiecień I, Kwiecień M. Gels, 2018, 4: 47

    Article  PubMed  PubMed Central  Google Scholar 

  25. Lin L, Zhang J. BMC Immunol, 2017, 18: 2

    Article  PubMed  PubMed Central  Google Scholar 

  26. Kumar A, Capua E, Kesharwani MK, Martin JML, Sitbon E, Waldeck DH, Naaman R. Proc Natl Acad Sci USA, 2017, 114: 2474–2478

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  27. Nemati A, Shadpour S, Querciagrossa L, Li L, Mori T, Gao M, Zannoni C, Hegmann T. Nat Commun, 2018, 9: 3908

    Article  PubMed  PubMed Central  Google Scholar 

  28. Bonner WA. Origins Life Evol Biosphere, 1991, 21: 59–111

    Article  CAS  Google Scholar 

  29. Feringa BL, van Delden RA. Angew Chem Int Ed, 1999, 38: 3418–3438

    Article  CAS  Google Scholar 

  30. Ma B, Bianco A. Nat Rev Mater, 2023, 8: 403–413

    Article  CAS  Google Scholar 

  31. Miyamoto T, Homma H. J Biochem, 2021, 170: 5–13

    Article  PubMed  CAS  Google Scholar 

  32. Suzuki M, Sujino T, Chiba S, Harada Y, Goto M, Takahashi R, Mita M, Hamase K, Kanai T, Ito M, Waldor MK, Yasui M, Sasabe J. Sci Adv, 2021, 7: eabd6480

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  33. Liu F, Cheng Z, Jiang Y, Gao L, Liu H, Liu H, Feng Z, Lu P, Yang W. Angew Chem Int Ed, 2022, 61: 7789–7793

    Google Scholar 

  34. Zhao X, Xu L, Sun M, Ma W, Wu X, Xu C, Kuang H. Nat Commun, 2017, 8: 2007

    Article  PubMed  PubMed Central  Google Scholar 

  35. Jiang S, Zeng Q, Zhao K, Liu J, Sun Q, Huang K, He Y, Zhang X, Wang H, Shi X, Feng C, Deng X, Wei Y. Adv Mater, 2022, 34: 2105136

    Article  CAS  Google Scholar 

  36. Yeom J, Guimaraes PPG, Ahn HM, Jung BK, Hu Q, McHugh K, Mitchell MJ, Yun CO, Langer R, Jaklenec A. Adv Mater, 2020, 32: 1903878

    Article  CAS  Google Scholar 

  37. Sun M, Xu L, Qu A, Zhao P, Hao T, Ma W, Hao C, Wen X, Colombari FM, de Moura AF, Kotov NA, Xu C, Kuang H. Nat Chem, 2018, 10: 821–830

    Article  PubMed  CAS  Google Scholar 

  38. Hou K, Zhao J, Wang H, Li B, Li K, Shi X, Wan K, Ai J, Lv J, Wang D, Huang Q, Wang H, Cao Q, Liu S, Tang Z. Nat Commun, 2020, 11: 4790

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  39. Xu L, Wang X, Wang W, Sun M, Choi WJ, Kim JY, Hao C, Li S, Qu A, Lu M, Wu X, Colombari FM, Gomes WR, Blanco AL, de Moura AF, Guo X, Kuang H, Kotov NA, Xu C. Nature, 2022, 601: 366–373

    Article  PubMed  CAS  Google Scholar 

  40. Liang K, Xue Y, Zhao B, Wen M, Xu Z, Sukhorukov G, Zhang L, Shang L. Small, 2023, 2304857

  41. Jiang H, Liu R, Wang L, Wang X, Zhang M, Lin S, Cao Z, Wu F, Liu Y, Liu J. Adv Mater, 2023, 35: 2208157

    Article  CAS  Google Scholar 

  42. Leal J, Smyth HDC, Ghosh D. Int J Pharm, 2017, 532: 555–572

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  43. Paone P, Cani PD. Gut, 2020, 69: 2232–2243

    Article  PubMed  CAS  Google Scholar 

  44. Johansson MEV, Sjövall H, Hansson GC. Nat Rev Gastroenterol Hepatol, 2013, 10: 352–361

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  45. Zhang T, Li Q, Cheng L, Buch H, Zhang F. Microb Biotechnol, 2019, 12: 1109–1125

    Article  PubMed  PubMed Central  Google Scholar 

  46. Nejman D, Livyatan I, Fuks G, Gavert N, Zwang Y, Geller LT, Rotter-Maskowitz A, Weiser R, Mallel G, Gigi E, Meltser A, Douglas GM, Kamer I, Gopalakrishnan V, Dadosh T, Levin-Zaidman S, Avnet S, Atlan T, Cooper ZA, Arora R, Cogdill AP, Khan MAW, Ologun G, Bussi Y, Weinberger A, Lotan-Pompan M, Golani O, Perry G, Rokah M, Bahar-Shany K, Rozeman EA, Blank CU, Ronai A, Shaoul R, Amit A, Dorfman T, Kremer R, Cohen ZR, Harnof S, Siegal T, Yehuda-Shnaidman E, Gal-Yam EN, Shapira H, Baldini N, Langille MGI, Ben-Nun A, Kaufman B, Nissan A, Golan T, Dadiani M, Levanon K, Bar J, Yust-Katz S, Barshack I, Peeper DS, Raz DJ, Segal E, Wargo JA, Sandbank J, Shental N, Straussman R. Science, 2020, 368: 973–980

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  47. Zhou S, Gravekamp C, Bermudes D, Liu K. Nat Rev Cancer, 2018, 18: 727–743

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  48. Hurt RC, Buss MT, Duan M, Wong K, You MY, Sawyer DP, Swift MB, Dutka P, Barturen-Larrea P, Mittelstein DR, Jin Z, Abedi MH, Farhadi A, Deshpande R, Shapiro MG. Nat Biotechnol, 2023, 41: 919–931

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  49. Lin L, Song J, Du Y, Wu Q, Gao J, Song Y, Yang C, Wang W. Angew Chem Int Ed, 2020, 59: 11923–11926

    Article  CAS  Google Scholar 

  50. Zhao E, Chen Y, Chen S, Deng H, Gui C, Leung CWT, Hong Y, Lam JWY, Tang BZ. Adv Mater, 2015, 27: 4931–4937

    Article  PubMed  CAS  Google Scholar 

  51. Wu M, Wu W, Duan Y, Liu X, Wang M, Phan CU, Qi G, Tang G, Liu B. Adv Mater, 2020, 32: 2005222

    Article  CAS  Google Scholar 

  52. Li Y, Hu X, Ding D, Zou Y, Xu Y, Wang X, Zhang Y, Chen L, Chen Z, Tan W. Nat Commun, 2017, 8: 15653

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  53. Geng Z, Cao Z, Liu R, Liu K, Liu J, Tan W. Nat Commun, 2021, 12: 6584

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Key Research and Development Program of China (2021YFA0909400), the National Natural Science Foundation of China (22102097, 52101289, 21875135), the Fundamental Research Funds for the Central Universities (2020JCPT02), the Innovative Research Team of High-Level Local Universities in Shanghai (SHSMU-ZDCX20210900), the Explorer Program of the Science and Technology Commission of Shanghai Municipality (21TS1400400), and the Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant Support (20181704).

Author information

Authors and Affiliations

  1. Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Institute of Molecular Medicine, State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China

    Hejin Jiang, Rui Liu, Ying Liu, Huilong Luo, Zhenping Cao & Jinyao Liu

Authors
  1. Hejin Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rui Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ying Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Huilong Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhenping Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jinyao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Huilong Luo, Zhenping Cao or Jinyao Liu.

Ethics declarations

Conflict of interest The authors declare no conflict of interest.

Additional information

Supporting information The supporting information is available online at chem.scichina.com and link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jiang, H., Liu, R., Liu, Y. et al. Chiral coating-mediated interactions of bacteria with diverse biointerfaces. Sci. China Chem. 66, 3594–3601 (2023). https://doi.org/10.1007/s11426-023-1880-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-023-1880-8

Search

Navigation