Skip to main content
SpringerLink
Log in

Enzymatic self-assembly/disassembly turns “ON”/“OFF” the mimetic hydrolytic activity of histidine nanofibers

  • Communications
  • Published:
Science China Chemistry Aims and scope Submit manuscript

Abstract

Mimetic enzymes are devised as alternates or supplements of natural enzymes in broad fields but regulating their activities in a switchable manner remains challenging. Herein, we proposed an enzymatic self-assembly/disassembly strategy to address this issue. A peptide molecule NapFFEYIH (YH) was rationally designed which, after self-assembling into nanofibers, lined up the histidine moieties to form active hydrolysis centers for mimicking hydrolase activity. Enzymatic dephosphorylation of NapF-FEYpIH (YpH) by alkaline phosphatase to yield YH also turned “ON” the hydrolase activity. In turn, phosphorylation of YH by phosphokinase epidermal growth factor receptor to yield YpH disassembled the nanofibers and thus turned the activity “OFF”. As such, the “ON”/“OFF” of the mimetic hydrolase activities could be regulated under physiological conditions through ALP/EGFR-mediated self-assembly/disassembly of histidine nanofibers. This work provides a feasible strategy for the on-demand fabrication of artificial enzymes with controllable and superior activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bell EL, Finnigan W, France SP, Green AP, Hayes MA, Hepworth LJ, Lovelock SL, Niikura H, Osuna S, Romero E, Ryan KS, Turner NJ, Flitsch SL. Nat Rev Methods Primers, 2021, 1: 46

    Article  CAS  Google Scholar 

  2. Peng G, Jin H, Liu F, Yang X, Sui P, Lin S. Sci China Chem, 2022, 65: 2444–2449

    Article  CAS  Google Scholar 

  3. Yang J, Wang H, Yin Z, Zhang S, Xu JF, Zhang X. Sci China Chem, 2022, 65: 2252–2259

    Article  CAS  Google Scholar 

  4. Sun X, Dong Y, Liu Y, Song N, Li F, Yang D. Sci China Chem, 2022, 65: 31–47

    Article  CAS  Google Scholar 

  5. Tang Q, Cao S, Ma T, Xiang X, Luo H, Borovskikh P, Rodriguez RD, Guo Q, Qiu L, Cheng C. Adv Funct Mater, 2021, 31: 2007475

    Article  CAS  Google Scholar 

  6. Liu X, Sun X, Liang G. Biomater Sci, 2021, 9: 315–327

    Article  PubMed  CAS  Google Scholar 

  7. Deng Y, Zhan W, Liang G. Adv Healthc Mater, 2021, 10: 2001211

    Article  CAS  Google Scholar 

  8. Zozulia O, Dolan MA, Korendovych IV. Chem Soc Rev, 2018, 47: 3621–3639

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  9. Gao Y, Zhao F, Wang Q, Zhang Y, Xu B. Chem Soc Rev, 2010, 39: 3425–3433

    Article  PubMed  CAS  Google Scholar 

  10. Rufo CM, Moroz YS, Moroz OV, Stöhr J, Smith TA, Hu X, DeGrado WF, Korendovych IV. Nat Chem, 2014, 6: 303–309

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  11. Xia T, Cheng X, Sun X, Liang G. ACS Mater Lett, 2022, 4: 1962–1966

    Article  CAS  Google Scholar 

  12. Ma M, Liu Z, Gao N, Pi Z, Du X, Ren J, Qu X. J Am Chem Soc, 2020, 142: 21702–21711

    Article  PubMed  CAS  Google Scholar 

  13. Makam P, Yamijala SSRKC, Tao K, Shimon LJW, Eisenberg DS, Sawaya MR, Wong BM, Gazit E. Nat Catal, 2019, 2: 977–985

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. Reja A, Afrose SP, Das D. Angew Chem Int Ed, 2020, 59: 4329–4334

    Article  CAS  Google Scholar 

  15. Makam P, Yamijala SSRKC, Bhadram VS, Shimon LJW, Wong BM, Gazit E. Nat Commun, 2022, 13: 1505

    Article  PubMed  PubMed Central  ADS  CAS  Google Scholar 

  16. Jian T, Zhou Y, Wang P, Yang W, Mu P, Zhang X, Zhang X, Chen CL. Nat Commun, 2022, 13: 3025

    Article  PubMed  PubMed Central  ADS  CAS  Google Scholar 

  17. Nothling MD, Xiao Z, Bhaskaran A, Blyth MT, Bennett CW, Coote ML, Connal LA. ACS Catal, 2018, 9: 168–187

    Article  Google Scholar 

  18. Zhang C, Shafi R, Lampel A, MacPherson D, Pappas CG, Narang V, Wang T, Maldarelli C, Ulijn RV. Angew Chem Int Ed, 2017, 56: 14511–14515

    Article  CAS  Google Scholar 

  19. Miao X, Cao W, Zheng W, Wang J, Zhang X, Gao J, Yang C, Kong D, Xu H, Wang L, Yang Z. Angew Chem IntEd, 2013, 52: 7781–7785

    Article  CAS  Google Scholar 

  20. Singh A, Joseph JP, Gupta D, Miglani C, Mavlankar NA, Pal A. Nanoscale, 2021, 13: 13401–13409

    Article  PubMed  CAS  Google Scholar 

  21. Zheng Z, Wang J, Chen P, Xie M, Zhang L, Hou Y, Zhang X, Jiang J, Wang J, Lu Q, Liang G. Nanoscale, 2016, 8: 15142–15146

    Article  PubMed  CAS  Google Scholar 

  22. Bah A, Vernon RM, Siddiqui Z, Krzeminski M, Muhandiram R, Zhao C, Sonenberg N, Kay LE, Forman-Kay JD. Nature, 2015, 519: 106–109

    Article  PubMed  ADS  CAS  Google Scholar 

  23. Chen Y, Zhang W, Ding Y, Liang C, Shi Y, Hu ZW, Wang L, Yang Z. Sci China Chem, 2021, 64: 1554–1559

    Article  CAS  Google Scholar 

  24. Micsonai A, Wien F, Kernya L, Lee YH, Goto Y, Réfrégiers M, Kardos J. Proc Natl Acad Sci USA, 2015, 112: E3095

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  25. Fleming S, Frederix PWJM, Ramos Sasselli I, Hunt NT, Ulijn RV, Tuttle T. Langmuir, 2013, 29: 9510–9515

    Article  PubMed  CAS  Google Scholar 

  26. Zheng Z, Sun H, Hu C, Li G, Liu X, Chen P, Cui Y, Liu J, Wang J, Liang G. Anal Chem, 2016, 88: 3363–3368

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the National Natural Science Foundation of China (22234002, 22204019, 82172097) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX22_0245).

Author information

Authors and Affiliations

  1. State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China

    Xianbao Sun, Manli Wang, Xiaoyang Liu, Wenjun Zhan, Qiaochu Jiang, Hai-Dong Xu & Gaolin Liang

  2. Analysis Center, Nanjing Medical University, Nanjing, 210029, China

    Guangqiong Xu & Fuqiang Wang

Authors
  1. Xianbao Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Manli Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoyang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wenjun Zhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guangqiong Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qiaochu Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hai-Dong Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fuqiang Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Gaolin Liang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Fuqiang Wang or Gaolin Liang.

Ethics declarations

Conflict of interest The authors declare no conflict of interest.

Additional information

Supporting information The supporting information is available online at chem.scichina.com and link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

Supporting Information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, X., Wang, M., Liu, X. et al. Enzymatic self-assembly/disassembly turns “ON”/“OFF” the mimetic hydrolytic activity of histidine nanofibers. Sci. China Chem. 67, 517–522 (2024). https://doi.org/10.1007/s11426-023-1788-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-023-1788-5

Search

Navigation