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CATase-immobilized hydrogel platform molded by photo-enzyme coupling polymerization for effectively preventing postoperative abdominal adhesion

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Abstract

Redox homeostasis, which is regulated by enzymes acting as regulatory valves, is crucial for maintaining the proper functioning of biomolecules and a stable microenvironment for physiological processes by modulating the homeostasis of reactive oxygen species (ROS). Antioxidant enzymes in biocatalysis are used in the prevention or treatment of oxidative stress-related disease by counteracting the harmful effects of ROS. However, designing a system that can efficiently immobilize antioxidant enzymes with high catalytic activity and stability is still challenging. Bioinspired by photo-biocatalysis, a novel and effective catalase (CATase)-immobilized hydrogel platform has been developed by the proposed photo-enzymatic coupled radical polymerization strategy of the visible light coupling with the porphyrin-centered CATase. The higher catalytic stability and activity can therefore be achieved due to the preferential polymerization of CATase-immobilized hydrogel platform with a favorable three-dimensional network of enhanced coupling efficacy between light and enzymes. The mechanisms of free radical-initiated polymerization as well as the antioxidant cycle in the photo-CATase coupling system have been explored. Intriguingly, the CATase-immobilized hydrogel platform affords an unprecedented antioxidant ability to scavenge ROS and provide an effective cellular protection mechanism against external oxidative stress. Additionally, the CATase-immobilized hydrogel platform can effectively prevent peritoneal adhesion by reducing the expression of inflammatory cytokines. Therefore, the novel CATase-immobilized hydrogel platform is a potential candidate for physical barriers that effectively prevent postoperative adhesion formation, offering a new anti-adhesion strategy for clinical applications.

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Acknowledgements

This work was supported by the National Science Fund for Distinguished Young Scholars (52125305), the National Natural Science Foundation of China (52173289, 52273147), and the Key Project of the First Demonstration Project (Artificial intelligence) of Interdisciplinary Joint Research of Tongji University (ZD-11-202151).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. School of Chemical Science and Engineering, Tongji University, Shanghai, 200092, China

    Min Hu, Mingyue Shi, Xia Wang, Yujing Tang, Dingze Zhou & Kai Pan

  2. Translational Research Institute of Brain and Brain-Like Intelligence, Shanghai Fourth People’s Hospital, School of Medicine, Tongji University, Shanghai, 200434, China

    Qigang Wang

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  1. Min Hu
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  2. Mingyue Shi
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  3. Xia Wang
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  6. Kai Pan
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Correspondence to Xia Wang or Qigang Wang.

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Supporting information The supporting information is available online at https://chem.scichina.com and https://link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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CATase-Immobilized Hydrogel Platform Molded by Photo-Enzyme Coupling Polymerization for Effectively Preventing Postoperative Abdominal Adhesion

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Hu, M., Shi, M., Wang, X. et al. CATase-immobilized hydrogel platform molded by photo-enzyme coupling polymerization for effectively preventing postoperative abdominal adhesion. Sci. China Chem. 66, 2664–2675 (2023). https://doi.org/10.1007/s11426-023-1697-3

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