Abstract
Programmable and precise regulation of genetic information is crucial in bioengineering and biomedicine; however, it remains challenging to implement this objective. Here we deployed DNA-functionalized MXenes as a smart delivery system for spatiotemporally controllable genome editing. The MXene nanovehicles rationally integrated photothermal effect with nucleic acid strand displacement reaction, thereby allowing for the binary logic gate-controlled release of Cas ribonucleoprotein complexes in response to different input patterns of NIR light and nucleic acids. This system was highly programmable and could be harnessed to construct 2-input (AND, OR, and N-IMPLY) and 3-input (AND/OR and N-IMPLY/OR) logic gates for precise gene editing in mammalian cells. Moreover, an AND logic gate-controlled delivery system achieved selective induction of tumor cell death in a xenograft mice model using tissue-penetrating NIR light and cancer-relevant microRNA as the inputting cues. Therefore, the MXene nanovehicles adopted both the external and endogenous signals as the stimuli to precisely control gene editing under logic computation, presenting a helpful strategy for therapeutic genome editing.
Similar content being viewed by others
References
Tang Y, Gao L, Feng W, Guo C, Yang Q, Li F, Le XC. Chem Soc Rev, 2021, 50: 11844–11869
Pickar-Oliver A, Gersbach CA. Nat Rev Mol Cell Biol, 2019, 20: 490–507
Liu G, Lin Q, Jin S, Gao C. Mol Cell, 2022, 82: 333–347
Makarova KS, Wolf YI, Iranzo J, Shmakov SA, Alkhnbashi OS, Brouns SJJ, Charpentier E, Cheng D, Haft DH, Horvath P, Moineau S, Mojica FJM, Scott D, Shah SA, Siksnys V, Terns MP, Venclovas Č, White MF, Yakunin AF, Yan W, Zhang F, Garrett RA, Backofen R, van der Oost J, Barrangou R, Koonin EV. Nat Rev Microbiol, 2020, 18: 67–83
Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. Science, 2012, 337: 816–821
Hsu PD, Lander ES, Zhang F. Cell, 2014, 157: 1262–1278
Doudna JA. Nature, 2020, 578: 229–236
Cai W, Wang M. Sci Bull, 2019, 64: 1841–1849
Glass Z, Lee M, Li Y, Xu Q. Trends Biotechnol, 2018, 36: 173–185
Wang P, Zhang L, Xie Y, Wang N, Tang R, Zheng W, Jiang X. Adv Sci, 2017, 4: 1700175
Yang X, Tang Q, Jiang Y, Zhang M, Wang M, Mao L. J Am Chem Soc, 2019, 141: 3782–3786
Zhou W, Cui H, Ying L, Yu XF. Angew Chem Int Ed, 2018, 57: 10268–10272
Li F, Song N, Dong Y, Li S, Li L, Liu Y, Li Z, Yang D. Angew Chem Int Ed, 2022, 61
Staahl BT, Benekareddy M, Coulon-Bainier C, Banfal AA, Floor SN, Sabo JK, Urnes C, Munares GA, Ghosh A, Doudna JA. Nat Biotechnol, 2017, 35: 431–434
Rouet R, Thuma BA, Roy MD, Lintner NG, Rubitski DM, Finley JE, Wisniewska HM, Mendonsa R, Hirsh A, de Oñate L, Compte Barrón J, McLellan TJ, Bellenger J, Feng X, Varghese A, Chrunyk BA, Borzilleri K, Hesp KD, Zhou K, Ma N, Tu M, Dullea R, McClure KF, Wilson RC, Liras S, Mascitti V, Doudna JA. J Am Chem Soc, 2018, 140: 6596–6603
Naeem M, Majeed S, Hoque MZ, Ahmad I. Cells, 2020, 9: 1608
Grünewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK. Nature, 2019, 569: 433–437
Li L, Yang Z, Zhu S, He L, Fan W, Tang W, Zou J, Shen Z, Zhang M, Tang L, Dai Y, Niu G, Hu S, Chen X. Adv Mater, 2019, 31: 1901187
Pan Y, Yang J, Luan X, Liu X, Li X, Yang J, Huang T, Sun L, Wang Y, Lin Y, Song Y. Sci Adv, 2019, 5: eaav7199
Lyu Y, He S, Li J, Jiang Y, Sun H, Miao Y, Pu K. Angew Chem Int Ed, 2019, 58: 18197–18201
Pu Y, Yin H, Dong C, Xiang H, Wu W, Zhou B, Du D, Chen Y, Xu H. Adv Mater, 2021, 33: 2104641
Liu S, Cheng Q, Wei T, Yu X, Johnson LT, Farbiak L, Siegwart DJ. Nat Mater, 2021, 20: 701–710
Cheng Q, Wei T, Farbiak L, Johnson LT, Dilliard SA, Siegwart DJ. Nat Nanotechnol, 2020, 15: 313–320
Wu J, Peng H, Lu X, Lai M, Zhang H, Le XC. Angew Chem Int Ed, 2021, 60: 11104–11109
Cai W, Luo T, Mao L, Wang M. Angew Chem Int Ed, 2021, 60: 8596–8606
Ryu JY, Won EJ, Lee HAR, Kim JH, Hui E, Kim HP, Yoon TJ. Biomaterials, 2020, 232: 119736
Manna D, Maji B, Gangopadhyay SA, Cox KJ, Zhou Q, Law BK, Mazitschek R, Choudhary A. Angew Chem Int Ed, 2019, 58: 6285–6289
Kim H, Lee WJ, Oh Y, Kang SH, Hur JK, Lee H, Song WJ, Lim KS, Park YH, Song BS, Jin YB, Jun BH, Jung C, Lee DS, Kim SU, Lee SH. Nucl Acids Res, 2020, 48: 8601–8616
Breinig M, Schweitzer AY, Herianto AM, Revia S, Schaefer L, Wendler L, Cobos Galvez A, Tschaharganeh DF. Nat Methods, 2019, 16: 51–54
Gangopadhyay SA, Cox KJ, Manna D, Lim D, Maji B, Zhou Q, Choudhary A. Biochemistry, 2019, 58: 234–244
Kempton HR, Goudy LE, Love KS, Qi LS. Mol Cell, 2020, 78: 184–191.e3
Rohaizad N, Mayorga-Martinez CC, Fojtů M, Latiff NM, Pumera M. Chem Soc Rev, 2021, 50: 619–657
VahidMohammadi A, Rosen J, Gogotsi Y. Science, 2021, 372: eabf1581
Huang K, Li Z, Lin J, Han G, Huang P. Chem Soc Rev, 2018, 47: 5109–5124
Xuan J, Wang Z, Chen Y, Liang D, Cheng L, Yang X, Liu Z, Ma R, Sasaki T, Geng F. Angew Chem Int Ed, 2016, 55: 14569–14574
Lin H, Gao S, Dai C, Chen Y, Shi J. J Am Chem Soc, 2017, 139: 16235–16247
Wang S, Chen Y, Li X, Gao W, Zhang L, Liu J, Zheng Y, Chen H, Shi J. Adv Mater, 2015, 27: 7117–7122
Tang W, Dong Z, Zhang R, Yi X, Yang K, Jin M, Yuan C, Xiao Z, Liu Z, Cheng L. ACS Nano, 2019, 13: 284–294
Wang S, Wei S, Wang S, Zhu X, Lei C, Huang Y, Nie Z, Yao S. Anal Chem, 2019, 91: 1651–1658
Wang S, Song W, Wei S, Zeng S, Yang S, Lei C, Huang Y, Nie Z, Yao S. Anal Chem, 2019, 91: 8622–8629
Li J, Green AA, Yan H, Fan C. Nat Chem, 2017, 9: 1056–1067
Wang M, He F, Li H, Yang S, Zhang J, Ghosh P, Wang HH, Nie Z. Nano Lett, 2019, 19: 2603–2613
Xin H, Namgung B, Lee LP. Nat Rev Mater, 2018, 3: 228–243
Peng H, Le C, Wu J, Li XF, Zhang H, Le XC. ACS Nano, 2020, 14: 2817–2826
Dong D, Ren K, Qiu X, Zheng J, Guo M, Guan X, Liu H, Li N, Zhang B, Yang D, Ma C, Wang S, Wu D, Ma Y, Fan S, Wang J, Gao N, Huang Z. Nature, 2016, 532: 522–526
Hall A, Lächelt U, Bartek J, Wagner E, Moghimi SM. Mol Ther, 2017, 25: 1476–1490
Degors IMS, Wang C, Rehman ZU, Zuhorn IS. Acc Chem Res, 2019, 52: 1750–1760
Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS. Nat Methods, 2016, 13: 1043–1049
Lucks JB, Qi L, Mutalik VK, Wang D, Arkin AP. Proc Natl Acad Sci USA, 2011, 108: 8617–8622
Rosenblum D, Gutkin A, Kedmi R, Ramishetti S, Veiga N, Jacobi AM, Schubert MS, Friedmann-Morvinski D, Cohen ZR, Behlke MA, Lieberman J, Peer D. Sci Adv, 2020, 6: eabc9450
Zhang P, Liu X, Abegg D, Tanaka T, Tong Y, Benhamou RI, Baisden J, Crynen G, Meyer SM, Cameron MD, Chatterjee AK, Adibekian A, Childs-Disney JL, Disney MD. J Am Chem Soc, 2021, 143: 13044–13055
Micura R, Höbartner C. Chem Soc Rev, 2020, 49: 7331–7353
Acknowledgements
This work was supported by the National Key Research and Development Program of China (2020YFA0907500), the National Natural Science Foundation of China (22034002, 21974038, 21725503, 22074034) and the Natural Science Foundation of Hunan Province (2022JJ20004).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest The authors declare no conflict of interest.
Additional information
Supporting information The supporting information is available online at https://chem.scichina.com and https://link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.
Rights and permissions
About this article
Cite this article
Wang, S., Zhang, Z., Tang, R. et al. Responsive MXene nanovehicles deliver CRISPR/Cas12a for boolean logic-controlled gene editing. Sci. China Chem. 65, 2318–2326 (2022). https://doi.org/10.1007/s11426-022-1376-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11426-022-1376-1