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De novo design of transmembrane nanopores

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Abstract

Transmembrane nanopores are structurally stable and biocompatible, and have various applications in molecular sensing and selective transport. The relationship between the function and structure is crucial for transmembrane nanopores, but it is still challenging to design and precisely tune the structure of conventional protein nanopores from scratch, albeit of abundant previous work on natural and bioengineered transmembrane protein nanopores. Therefore, numerous types of artificial transmembrane nanopores that can be de novo designed are rapidly under development, such as molecular nanopores, peptide nanopores, and DNA origami nanopores. In this review, we compare different building blocks of “bottom-up” built nanopores in terms of construction methods, structures and applications, and also describe important advances in de novo designed proteins from the perspective of theoretical simulations as well as an outlook for artificial intelligence-assisted nanopore design.

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Acknowledgements

Jiandong Feng acknowledges the support from the National Natural Science Foundation of China (21974123), the Natural Science Foundation of Zhejiang Province (LR20B050002), and the Fundamental Research Funds for the Central Universities (2022KYY5060320001).

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Authors and Affiliations

  1. Laboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang University, Hangzhou, 310027, China

    Dan Qiao, Yuang Chen, Haojing Tan & Jiandong Feng

  2. Institute of Quantitative Biology, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

    Ruhong Zhou

  3. Zhejiang Lab, Hangzhou, 310000, China

    Jiandong Feng

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  1. Dan Qiao
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  2. Yuang Chen
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  3. Haojing Tan
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  4. Ruhong Zhou
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Correspondence to Jiandong Feng.

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Qiao, D., Chen, Y., Tan, H. et al. De novo design of transmembrane nanopores. Sci. China Chem. 65, 2122–2143 (2022). https://doi.org/10.1007/s11426-022-1354-5

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