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Cleavable Cys labeling directed Lys site-selective stapling and single-site modification

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An Erratum to this article was published on 20 September 2022

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Abstract

Site-selective modification of peptide/protein is a vital approach to disclose post-translational modifications (PTMs) and plays a crucial role in chemical biology, as well as drug development. Compared with synthetic and chemical biology methods, chemical modification of native peptide/protein provides a more versatile approach to achieve late-stage diversification for functional studies. Lysine featured high nucleophilicity, frequency, and solvent accessibility, making its site-selective modification important but elusive. Herein, we reported a visible-light-driven and Cys-directed Lys site-selective stapling approach for peptide/protein. By cleavable Cys anchoring, site-selective Lys single-site modification was achieved, and this method could be applied to multi-functionalization.

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Acknowledgements

This work was supported by Guangdong Natural Science Funds for Distinguished Young Scholar (2018B030306017), the National Natural Science Foundation of China (22077144), Guangdong Provincial Key Laboratory of Chiral Molecule and Drug Discovery (2019B030301005), and Key Research and Development Program of Guangdong Province (2020B1111110003).

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Authors and Affiliations

  1. Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China

    Jian Li, Qi-Long Hu, Zhendong Song, Albert S. C. Chan & Xiao-Feng Xiong

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  1. Jian Li
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  2. Qi-Long Hu
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  3. Zhendong Song
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  4. Albert S. C. Chan
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  5. Xiao-Feng Xiong
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Corresponding authors

Correspondence to Albert S. C. Chan or Xiao-Feng Xiong.

Additional information

Supporting information The supporting information is available online at http://chem.scichina.com and http://link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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The authors declare no conflict of interest.

The online version of the original article can be found at https://doi.org/10.1007/s11426-022-1393-1

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Li, J., Hu, QL., Song, Z. et al. Cleavable Cys labeling directed Lys site-selective stapling and single-site modification. Sci. China Chem. 65, 1356–1361 (2022). https://doi.org/10.1007/s11426-022-1252-7

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  • DOI: https://doi.org/10.1007/s11426-022-1252-7

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