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Dispen-Seq: a single-microparticle dispenser based strategy towards flexible cell barcoding for single-cell RNA sequencing

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Abstract

Single-cell RNA sequencing (scRNA-seq) has become one of the most powerful tools to understand the heterogeneity of biological systems. While barcoding strategies have revolutionized the field of high-throughput scRNA-seq, it is still challenging to achieve highly efficient, direct and universal cell barcoding with cost-effectiveness and minimal sample loss. Herein, a single micro-particle dispenser approach for rapid single barcode bead/cell manipulation and pairing, enabling highly efficient cell barcoding for scRNA-seq (Dispen-Seq) was developed. Notably, Dispen-Seq provides a versatile platform which can enrich cell subgroups of interest while unlimited by input sample amounts, and can respond to changes in sample composition with high resolution and reproducibility. It is anticipated that Dispen-Seq will increase the scope of scRNA-seq from academic research to practical applications.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2018YFC1602900), the National Natural Science Foundation of China (21927806, 21735004, 222022409, 21874089, 21705024, 21775128) and the Program for Changjiang Scholars and Innovative Research Team in University (IRT13036).

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Physical Chemistry of Solid Surfaces, Key Laboratory for Chemical Biology of Fujian Province, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China

    Tian Tian, Yingwen Chen, Yunpeng Bi, Xingrui Li, MingXuan Gao, Xuebing Zhang, Weidong Ruan, Xing Xu, Zhi Zhu, Yanling Song & Chaoyong Yang

  2. Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, China

    Jia Song, Lingling Wu & Chaoyong Yang

  3. Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China

    Tian Tian

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  1. Tian Tian
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  2. Yingwen Chen
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  3. Yunpeng Bi
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Correspondence to Yanling Song or Chaoyong Yang.

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Conflict of interest The authors declare no conflict of interest.

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11426_2020_9925_MOESM1_ESM.docx

Dispen-Seq: A Single-Microparticle Dispenser Based Strategy Towards Flexible Cell Barcoding for Single-Cell RNA Sequencing

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Tian, T., Chen, Y., Bi, Y. et al. Dispen-Seq: a single-microparticle dispenser based strategy towards flexible cell barcoding for single-cell RNA sequencing. Sci. China Chem. 64, 650–659 (2021). https://doi.org/10.1007/s11426-020-9925-8

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  • DOI: https://doi.org/10.1007/s11426-020-9925-8

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