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A fluorescent probe operating under weak acidic conditions for the visualization of HOCl in solid tumors in vivo

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Abstract

Levels of reactive oxygen species (ROS) in cancer cells or in the tumor microenvironment differ noticeably from those in normal cells and cellular microenvironments because ROS play important roles in all aspects of tumor physiology. However, due to the lack of adequate tools, it is difficult to study the relationship between ROS, especially certain types of ROS (e.g., HOCl), and cancer. We report herein an HOCl-specific fluorescent probe, FDOCl-20, containing a thiocarbamide group as a receptor, for the visualization of HOCl in solid tumors in vivo. This probe displays high selectivity and sensitivity to HOCl, and is appropriate for use in acidic conditions, including the tumor microenvironment. Using FDOCl-20 as a tool, we can visualize HOCl in solid tumors in vivo. Importantly, the fluorescent intensity of FDOCl-20 is proportional to tumor volume. Thus, FDOCl-20 is a useful tool to investigate the relationship between HOCl and the physiological processes of tumors.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21877013, 21671043) and the Initial Research Funds for Young Teachers of Donghua University.

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Authors and Affiliations

  1. State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Donghua University, Shanghai, 201620, China

    Peng Wei & Tao Yi

  2. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, 201620, China

    Peng Wei & Tao Yi

  3. Department of Chemistry, Fudan University, Shanghai, 200438, China

    Lingyan Liu, Wei Yuan, Jiajia Yang, Ruohan Li & Tao Yi

Authors
  1. Peng Wei
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  2. Lingyan Liu
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  3. Wei Yuan
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  4. Jiajia Yang
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  5. Ruohan Li
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  6. Tao Yi
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Correspondence to Wei Yuan or Tao Yi.

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Wei, P., Liu, L., Yuan, W. et al. A fluorescent probe operating under weak acidic conditions for the visualization of HOCl in solid tumors in vivo. Sci. China Chem. 63, 1153–1158 (2020). https://doi.org/10.1007/s11426-020-9737-y

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  • DOI: https://doi.org/10.1007/s11426-020-9737-y

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