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Preparation of biomimetic gene hydrogel via polymerase chain reaction for cell-free protein expression

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Abstract

Deoxyribonucleic acid (DNA) hydrogels, a three-dimensional (3D) network made from DNA chains, have attracted great attention because of its molecular programmability, excellent biocompatibility and wide biomedical applications. Construction of hydrogel incorporating genetic function is still a challenge because of the limitations in available preparation methods. Herein, we develop a polymerase chain reaction (PCR) based strategy to construct gene integrated hydrogel to mimic the biofunction of nucleus zone. DNA primers were chemically modified by methacrylamide, which were used as modular primers in PCR to hybridize with template plasmid DNA, yielding methacrylamide functionalized gene (Acry-gene). Afterwards, Acry-gene was chemically cross-linked and compressed via free radical polymerization of terminal group methacrylamide to form a three-dimensional gene network, namely gene hydrogel. The gene hydrogel retained the genetic function and expressed protein successfully in a cell free protein expression system. This work provides a general approach for the construction of biofunctional gene hydrogel which mimics bioprocesses, showing great potential in biomedicine and biomimetic fields.

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References

  1. Yang D, Hartman MR, Derrien TL, Hamada S, An D, Yancey KG, Cheng R, Ma M, Luo D. Acc Chem Res, 2014, 47: 1902–1911

    Article  CAS  Google Scholar 

  2. Wang F, Liu X, Willner I. Angew Chem Int Ed, 2015, 54: 1098–1129

    Article  CAS  Google Scholar 

  3. Song P, Ye D, Song S, Wang L, Zuo X. Prog Chem, 2016, 28: 628–636

    Google Scholar 

  4. Chen J, Yuan S, Song Y, Ma Q, Qi X, Wu Z. J Int Pharm Res, 2017, 44: 935–940, 946

    Google Scholar 

  5. Shao Y, Li C, Zhou X, Wu F, Jia H, Wang Y, Liu D. Polym Bull, 2015, 9: 100–108

    Google Scholar 

  6. Pei H, Zuo X, Zhu D, Huang Q, Fan C. Acc Chem Res, 2014, 47: 550–559

    Article  CAS  Google Scholar 

  7. Seeman NC. Nature, 2003, 421: 427–431

    Article  Google Scholar 

  8. Storhoff JJ, Mirkin CA. Chem Rev, 1999, 99: 1849–1862

    Article  CAS  Google Scholar 

  9. Geng J, Yao C, Kou X, Tang J, Luo D, Yang D. Adv Healthcare Mater, 2018, 7: 1700998

    Article  Google Scholar 

  10. Kahn JS, Hu Y, Willner I. Acc Chem Res, 2017, 50: 680–690

    Article  CAS  Google Scholar 

  11. Bian B, Zhang YY, Dong YC, Wu F, Wang C, Wang S, Xu Y, Liu DS. Sci China Chem, 2018, 61: 1568–1571

    Article  CAS  Google Scholar 

  12. Liu Y, Hu X, Fu T, Wang R, Tan W. Sci China Chem, 2019, 62: 407–408

    Article  CAS  Google Scholar 

  13. Shao Y, Jia H, Cao T, Liu D. Acc Chem Res, 2017, 50: 659–668

    Article  CAS  Google Scholar 

  14. Zinchenko A, Che Y, Taniguchi S, Lopatina LI, Sergeyev VG, Murata S. J Nanopart Res, 2016, 18: 179

    Article  Google Scholar 

  15. Mao X, Chen G, Wang Z, Zhang Y, Zhu X, Li G. Chem Sci, 2018, 9: 811–818

    Article  CAS  Google Scholar 

  16. Caliari SR, Burdick JA. Nat Methods, 2016, 13: 405–414

    Article  CAS  Google Scholar 

  17. Cangialosi A, Yoon CK, Liu J, Huang Q, Guo J, Nguyen TD, Gracias DH, Schulman R. Science, 2017, 357: 1126–1130

    Article  CAS  Google Scholar 

  18. Wu Y, Wang D, Willner I, Tian Y, Jiang L. Angew Chem Int Ed, 2018, 57: 7790–7794

    Article  CAS  Google Scholar 

  19. Yang L, Yao C, Li F, Dong Y, Zhang Z, Yang D. Small, 2018, 14: 1800185

    Article  Google Scholar 

  20. Nöll T, Wenderhold-Reeb S, Schönherr H, Nöll G. Angew Chem Int Ed, 2017, 56: 12004–12008

    Article  Google Scholar 

  21. Cheng E, Xing Y, Chen P, Yang Y, Sun Y, Zhou D, Xu L, Fan Q, Liu D. Angew Chem Int Ed, 2009, 48: 7660–7663

    Article  CAS  Google Scholar 

  22. Li C, Faulkner-Jones A, Dun AR, Jin J, Chen P, Xing Y, Yang Z, Li Z, Shu W, Liu D, Duncan RR. Angew Chem Int Ed, 2015, 54: 3957–3961

    Article  CAS  Google Scholar 

  23. Wang J, Chao J, Liu H, Su S, Wang L, Huang W, Willner I, Fan C. Angew Chem Int Ed, 2017, 56: 2171–2175

    Article  CAS  Google Scholar 

  24. Um SH, Lee JB, Park N, Kwon SY, Umbach CC, Luo D. Nat Mater, 2006, 5: 797–801

    Article  CAS  Google Scholar 

  25. Park N, Um SH, Funabashi H, Xu J, Luo D. Nat Mater, 2009, 8: 432–437

    Article  CAS  Google Scholar 

  26. Lee JB, Peng S, Yang D, Roh YH, Funabashi H, Park N, Rice EJ, Chen L, Long R, Wu M, Luo D. Nat Nanotech, 2012, 7: 816–820

    Article  CAS  Google Scholar 

  27. Xing Y, Cheng E, Yang Y, Chen P, Zhang T, Sun Y, Yang Z, Liu D. Adv Mater, 2011, 23: 1117–1121

    Article  CAS  Google Scholar 

  28. Xu W, Huang Y, Zhao H, Li P, Liu G, Li J, Zhu C, Tian L. Chem Eur J, 2017, 23: 18276–18281

    Article  CAS  Google Scholar 

  29. Huang Y, Xu W, Liu G, Tian L. Chem Commun, 2017, 53: 3038–3041

    Article  CAS  Google Scholar 

  30. Li J, Zheng C, Cansiz S, Wu C, Xu J, Cui C, Liu Y, Hou W, Wang Y, Zhang L, Teng I, Yang HH, Tan W. J Am Chem Soc, 2015, 137: 1412–1415

    Article  CAS  Google Scholar 

  31. Bartlett JMS, Stirling D. Methods Mol Biol, 2003, 226: 3–6

    CAS  PubMed  Google Scholar 

  32. Eguchi Y, Kato T, Tanaka T, Maruyama T. Chem Commun, 2017, 53: 5802–5805

    Article  CAS  Google Scholar 

  33. Hartman MR, Yang D, Tran TNN, Lee K, Kahn JS, Kiatwuthinon P, Yancey KG, Trotsenko O, Minko S, Luo D. Angew Chem Int Ed, 2013, 52: 8699–8702

    Article  CAS  Google Scholar 

  34. Yang D, Peng S, Hartman MR, Gupton-Campolongo T, Rice EJ, Chang AK, Gu Z, Lu GQM, Luo D. Sci Rep, 2013, 3: 3165

    Article  Google Scholar 

  35. Perez JG, Stark JC, Jewett MC. Cold Spring Harb Perspect Biol, 2016, 8: a023853

    Article  Google Scholar 

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21621004, 21575101, 21622404).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Frontier Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300350, China

    Feng Li, Wenting Yu, Xue Zhang, Xiaocui Guo, Xihan Xu, Xiaolei Sun & Dayong Yang

Authors
  1. Feng Li
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  2. Wenting Yu
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  3. Xue Zhang
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  4. Xiaocui Guo
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  5. Xihan Xu
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  6. Xiaolei Sun
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  7. Dayong Yang
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Correspondence to Dayong Yang.

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Conflict of interest The authors declare that they have no conflict of interest.

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Li, F., Yu, W., Zhang, X. et al. Preparation of biomimetic gene hydrogel via polymerase chain reaction for cell-free protein expression. Sci. China Chem. 63, 99–106 (2020). https://doi.org/10.1007/s11426-019-9617-7

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