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Science China Chemistry

, Volume 61, Issue 7, pp 871–878 | Cite as

Using MALDI-TOF MS coupled with a high-mass detector to directly analyze intact proteins in thyroid tissues

  • Shan-Shan Wang
  • Yun-Jun Wang
  • Jing Zhang
  • Jun Xiang
  • Tuan-Qi Sun
  • Yin-Long Guo
Articles

Abstract

Protein analysis is vital for biological and clinical research, but the measurement of unseparated, intact and high-mass proteins is also a challenging task by mass spectrometry-based methods. Here, we present a protocol for rapid and high-throughput analysis of intact proteins in tissue samples using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with a high-mass detector platform. The method involves tissue specimens that undergo a simple protein extraction before MALDI-MS analysis. Using this method, the high abundance proteins in human thyroid carcinoma and paracarcinoma tissues were successfully investigated, and the mass spectra of the tissues of the 30 illustrated thyroid cancers showed remarkable differences. The peak intensity revealed a significant increase in human albumin in thyroid carcinoma tissues (p<0.05). To validate the feasibility and credibility of this method, label-free proteomics quantitative analysis and Western blotting were used to relatively quantify the proteins in these tissues. Those results demonstrated a nearly 3-fold difference in human albumin levels between thyroid carcinoma and para-carcinoma tissues, which were consistent with the results of our method. The advantages of our method are easy sample handling, remarkable reproducibility and the ability to analyze high-mass proteins without digestion, which make them have the potential to be used in biological research and in clinical practice.

Keywords

matrix-assisted laser desorption ionization time of flight mass spectrometry intact proteins analysis thyroid carcinoma high-mass detector 

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Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (21672250), Chinese Academy of Sciences (YZ201544), National Key Technology Support Program (2015BAK45B01) and the Shanghai Municipal Planning Commission of Science and Research Fund for Young Scholar (20154Y0050).

Supplementary material

11426_2017_9230_MOESM1_ESM.docx (1 mb)
Using MALDI-TOF MS coupled with a high-mass detector to directly analyze intact proteins in thyroid tissues
11426_2017_9230_MOESM2_ESM.xlsx (225 kb)
Suplementary Table S-5. Proteins Identified in thyroid carcinoma tissues
11426_2017_9230_MOESM3_ESM.xlsx (164 kb)
Suplementary Table S-6. Proteins Identified in para-carcinoma tissues

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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.State Key Laboratory of Organometallic Chemistry and National Center for Organic Mass Spectrometry in Shanghai, Center for Excellence in Molecular Synthesis, Shanghai Institute of Organic Chemistry, University of Chinese Academy of SciencesChinese Academy of SciencesShanghaiChina
  2. 2.Department of Head and Neck SurgeryFudan University Shanghai Cancer CenterShanghaiChina
  3. 3.Department of Oncology, Shanghai Medical CollegeFudan UniversityShanghaiChina

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