Science China Chemistry

, Volume 61, Issue 4, pp 393–396 | Cite as

An ultrasensitive aptasensor for prostate specific antigen assay based on Exonuclease T-aided cyclic cleavage

  • Guangxing Liu
  • Tian Zhang
  • Yuguo Tang
  • Peng Miao


An ultrasensitive electrochemical aptasensor is presented for prostate specific antigen (PSA) detection. DNA tetrahedronaptamer is designed, which not only facilitates the molecular self-assembly events, but also improves the recognition efficiency between PSA and aptamer sequence on the electrode interface. The DNA conformation on top of DNA tetrahedron changes accordingly, which can be further digested by Exonuclease T (Exo T), a type of single-strand specific nuclease. Electrochemical species are removed synchronously and the initial PSA level can thus be determined. A linear range from 0.5 pg mL−1 to 50 ng mL−1 is achieved with the limit of detection (LOD) as low as 0.15 pg mL−1. Moreover, this proposed method is highly selective and is successfully applied to determine PSA in human serum samples.


prostate specific antigen aptamer DNA tetrahedron Exonuclease T square wave voltammetry. 


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This work was supported by the National Natural Science Foundation of China (81771929) and China Postdoctoral Science Foundation (2017M611911).

Supplementary material

11426_2017_9203_MOESM1_ESM.pdf (372 kb)
An ultrasensitive aptasensor for prostate specific antigen assay based on Exonuclease T-aided cyclic cleavage


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Copyright information

© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Guangxing Liu
    • 1
    • 2
  • Tian Zhang
    • 1
    • 2
  • Yuguo Tang
    • 1
  • Peng Miao
    • 1
    • 2
  1. 1.CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and TechnologyChinese Academy of SciencesSuzhouChina
  2. 2.University of Science and Technology of ChinaHefeiChina

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