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Nanocarrier based on the assembly of protein and antisense oligonucleotide to combat multidrug resistance in tumor cells

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Abstract

Chemotherapy-induced multi-drug resistance (MDR) in tumors poses a huge challenge for clinical treatment of tumors. The downregulation of the multi-drug resistance relative protein, represented by P-glycoprotein (P-gp), can reverse MDR of cancer cells. In this study, we developed doxorubicin-loading nanocarrier based on the assembly of protein and antisense oligonucleotide (ASO) to combat MDR of cancer cells. The data demonstrate that the nanocarrier can efficiently deliver ASO to cytoplasm and downregulate the P-glycoprotein expression, subsequently improving the therapeutic effects of Dox in doxorubicin-resistant MCF-7/ADR cancer cells. The preparation is simple and effective, providing a powerful tool for gene delivery. Therefore, our nanocarrier shows high promise in cancer treatment.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21325520, 21327009, 21405041, J1210040), the Foundation for Innovative Research Groups of National Natural Science Foundation of China (21521063), and the Science and Technology Project of Hunan Province (2016RS2009, 2016WK2002).

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Authors and Affiliations

  1. Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Life Sciences, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, 410082, China

    Xuan Yu, Liang Gong, Jing Zhang, Zilong Zhao, Xiaobing Zhang & Weihong Tan

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  1. Xuan Yu
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  2. Liang Gong
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  3. Jing Zhang
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  4. Zilong Zhao
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  5. Xiaobing Zhang
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  6. Weihong Tan
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Correspondence to Zilong Zhao, Xiaobing Zhang or Weihong Tan.

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Yu, X., Gong, L., Zhang, J. et al. Nanocarrier based on the assembly of protein and antisense oligonucleotide to combat multidrug resistance in tumor cells. Sci. China Chem. 60, 1318–1323 (2017). https://doi.org/10.1007/s11426-017-9108-5

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  • DOI: https://doi.org/10.1007/s11426-017-9108-5

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