Abstract
Carbon nanotubes (CNTs) have been developed for medical and biotechnological applications in the past decades. Their widespread applications make it important to understand their potential hazards to human and the environment. In this study, the possible toxicological effects of the oxidized multi-walled carbon nanotubes (O-MWCNTs) were assessed on RAW 264.7 macrophages in vitro. Several toxicological endpoints, such as cell viability, the release of LDH and IL-8, GSH/GSSG ratio, intracellular calcium concentration and ultrastructural changes in cell morphology, were carried out. The results showed that O-MWCNTs had very limited effects on oxidative stress, cellular toxicity and apoptosis. Transmission electron microscope clearly demonstrates RAW 264.7 macrophages engulfed plenty of O-MWCNTs, and some of them resided in the cytoplasm, while the morphology was not altered by O-MWCNTs. As the control, the pristine MWCNTs (p-MWCNTs) show higher cytotoxicity than O-MWCNTs, damaging cell viability and inducing cell apoptosis. All these toxicological data are of benefit to more wide applications of O-MWCNTs in the future.
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Battigelli A, Menard-Moyon C, Da Ros T, Prato M, Bianco A. Adv Drug Deliver Rev, 2013, 65: 1899–1920
Li R, Wu R, Wu M, Zou H, Ma H, Yang L, Le XC. Electrophoresis, 2009, 30: 1906–1912
Saito N, Haniu H, Usui Y, Aoki K, Hara K, Takanashi S, Shimizu M, Narita N, Okamoto M, Kobayashi S, Nomura H, Kato H, Nishimura N, Taruta S, Endo M. Chem Rev, 2014, 114: 6040–6079
Yang ST, Wang H, Meziani MJ, Liu Y, Wang X, Sun YP. Biomacromolecules, 2009, 10: 2009–2012
Yang ST, Luo JB, Zhou QH, Wang HF. Theranostics, 2012, 2: 271–282
Nimmagadda A, Thurston K, Nollert MU, McFetridge PS. J Biomed Mater Res A, 2006, 76: 614–625
Garibaldi S, Brunelli C, Bavastrello V, Ghigliotti G, Nicolini C. Nanotechnology, 2006, 17:391
Cui D, Tian F, Ozkan CS, Wang M, Gao H. Toxicol Lett, 2005, 155: 73–85
Jia G, Wang H, Yan L, Wang X, Pei R, Yan T, Zhao YL, Guo X. Environ Sci Technol, 2005, 39: 1378–1383
Sharma CS, Sarkar S, Periyakaruppan A, Barr J, Wise K, Thomas R, Wilson NL, Ramesh GT. J Nanosci Nanotechnol, 2007, 7: 2466–2472
VanHandel M, Alizadeh D, Zhang L, Kateb B, Bronikowski M, Manohara H, Badie B. J Neuroimmunol, 2009, 208: 3–9
Wirnitzer U, Herbold B, Voetz M, Ragot J. Toxicol Lett, 2009, 186: 160–165
Dumortier H, Lacotte S, Pastorin G, Marega R, Wu W, Bonifazi D, Briand JP, Prato M, Muller S, Bianco A. Nano Lett, 2006, 6: 1522–1528
Figarol A, Pourchez J, Boudard D, Forest V, Tulliani JM, Lecompte JP, Cottier M, Bernache-Assollant D, Grosseau P. J Nanopart Res, 2014, 16: 2507
Yu GC, Li JY, Yu W, Han CY, Mao ZW, Gao CY, Huang FH. Adv Mater, 2013, 25: 6373–6379
Abe S, Itoh S, Hayashi D, Kobayashi T, Kiba T, Akasaka T, Uo M, Yawaka Y, Ato SI, Watari F, Takada T. J Nanosci Nanotechnol, 2012, 12: 700–706
Oberdörster G, Stone V, Donaldson K. Nanotoxicology, 2007, 1: 2–25
Jones CF, Grainger DW. Adv Drug Deliver Rev, 2009, 61: 438–456
Treumann S, Ma-Hock L, Groters S, Landsiedel R, van Ravenzwaay B. Toxicol Sci, 2013, 134: 103–110
Luanpitpong S, Wang LY, Rojanasakul Y. Nanomedicine, 2014, 9: 895–912
Deng X, Jia G, Wang H, Sun H, Wang X, Yang S, Wang T, Liu YF. Carbon, 2007, 45: 1419–1424
Deng X, Wu F, Liu Z, Luo M, Li L, Ni Q, Jiao Z, Wu MH, Liu YF. Carbon, 2009, 47: 1421–1428
Deng X, Yang S, Nie H, Wang H, Liu Y. Nanotechnology, 2008, 19: 075101
Ji Z, Zhang D, Li L, Shen X, Deng X, Dong L, Wu MH, Liu YF. Nanotechnology, 2009, 20: 445101
Liu Z, Davis C, Cai W, He L, Chen X, Dai H. Proc Natl Acad Sci USA, 2008, 105: 1410–1415
Schipper ML, Nakayama-Ratchford N, Davis CR, Kam NW, Chu P, Liu Z, Sun X, Dai H, Gambhir SS. Nat Nanotechnol, 2008, 3: 216–221
Brown DM, Stone V, Findlay P, MacNee W, Donaldson K. Occup Environ Med, 2000, 57: 685–691
Hirano S, Kanno S, Furuyama A. Toxicol Appl Pharm, 2008, 232: 244–251
Bottini M, Bruckner S, Nika K, Bottini N, Bellucci S, Magrini A, Bergamaschi A, Mustelin T. Toxicol Lett, 2006, 160: 121–126
Helland A, Wick P, Koehler A, Schmid K, Som C. Environ Health Perspect, 2007, 115: 1125–1231
Porter AE, Gass M, Muller K, Skepper JN, Midgley PA, Welland M. Nat Nanotechnol, 2007, 2: 713–717
Porter AE, Gass M, Bendall JS, Muller K, Goode A, Skepper JN, Midgley PA, Welland M. ACS Nano, 2009, 3: 1485–1492
Braakhuis HM, Park M, Gosens I, De Jong WH, Cassee FR. Part Fibre Toxicol, 2014, 11: 18
Rahman I, Yang SR, Biswas SK. Antioxid Redox Signal, 2006, 8: 681–689
Gasser M, Wick P, Clift MJD, Blank F, Diener L, Yan B, Gehr P, Krug HF, Rothen-Rutishauser B. Part Fibre Toxicol, 2012, 9: 17
Brown DM, Donaldson K, Borm PJ, Schins RP, Dehnhardt M, Gilmour P, Jimenez LA, Stone V. Am J Physiol Lung C, 2004, 286: 344–353
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Luo, M., Chen, P., Wang, J. et al. The cytotoxicity of oxidized multi-walled carbon nanotubes on macrophages. Sci. China Chem. 59, 918–926 (2016). https://doi.org/10.1007/s11426-016-5595-y
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DOI: https://doi.org/10.1007/s11426-016-5595-y