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Electrochemiluminescence of metal-organic complex nanowires based on graphene-Nafion modified electrode for biosensing application

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Abstract

In this work, we chose tris(2,2′-bipyridyl)ruthenium(II)hexafluorophosphate (Ru(bpy)3(PF6)2), a metal-organic complex material, to prepare nanowires, which were subsequently applied for the construction of electrochemiluminescence (ECL) biosensor by immobilizing them onto a glassy carbon electrode (GCE) with graphene-Nafion composite films. The graphene therein, being a two-dimensional carbon nanomaterial with outstanding electronic properties, can obviously improve the conductivity of the Nafion film, as well as enhance the electrochemical signal and ECL intensity of the Ru(bpy)3(PF6)2 nanowires (RuNWs) at low graphene concentration. The developed biosensor exhibited excellent ECL stability with tripropylamine (TPrA) as co-reactant. The ECL biosensor exhibited high sensitive ECL response in a wide linear range and low detection limit for the detection of proline. It is considered that the oxidation products of proline would be responsible for the ECL enhancement. The large electro-active area of the nanowires and the enhancement effect of the graphene played critical roles in the high detection performance of the ECL biosensor. The results demonstrated herein may provide a useful enlightenment for the design of more sensitive ECL biosensors.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21406053, 21521062), the Ministry of Science and Technology of China (2012YQ120060), Natural Science Foundation of Hebei Province (B2016208082), Science and Technology Research Projects in Hebei Universities (QN2015243, YQ2014015), and Five Platform Open Fund Projects of Hebei University of Science and Technology (2014YY25).

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Authors and Affiliations

  1. College of Chemistry and Pharmaceutical Engineering, Hebei University of Science and Technology, Shijiazhuang, 050018, China

    Qing Li, Caihong Kang, Kunjie Li & Aibing Chen

  2. Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

    Wei Zhang & Yong-Sheng Zhao

  3. Hebei Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science and Technology, Shijiazhuang, 050018, China

    Qing Li, Caihong Kang, Kunjie Li & Aibing Chen

Authors
  1. Qing Li
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  2. Caihong Kang
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  3. Kunjie Li
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  4. Aibing Chen
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  5. Wei Zhang
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  6. Yong-Sheng Zhao
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Correspondence to Qing Li or Yong-Sheng Zhao.

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11426_2016_457_MOESM1_ESM.pdf

Electrochemiluminescence of Metal-Organic Complex Nanowires Based on Graphene-Nafion Modified Electrode for Biosensing Application

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Li, Q., Kang, C., Li, K. et al. Electrochemiluminescence of metal-organic complex nanowires based on graphene-Nafion modified electrode for biosensing application. Sci. China Chem. 60, 642–648 (2017). https://doi.org/10.1007/s11426-016-0457-9

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  • DOI: https://doi.org/10.1007/s11426-016-0457-9

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