Science China Chemistry

, Volume 59, Issue 6, pp 719–723 | Cite as

Imaging cellular distribution of fluorescent supramolecular nanofibers

  • Jingyu Wang
  • Jing Zheng
  • Yanbin Cai
  • Jinxin Zheng
  • Jie Gao
  • Qingqiu Gong
  • Zhimou Yang


In this study, we used the 4-nitro-2,1,3-benzoxadiazole (NBD) as an aromatic capping group for a peptide to construct the supramolecular nanofibers. Taking the advantage of the fluorescence property of NBD, we could directly observe the cellular distribution of the self-assembled nanofibers. We found that the distributions of the nanofibers of NBD-FFETIGGY are different in four mammalian cells and two plant cells. The nanofibers are mainly located at the surface of two mammalian cells and one plant cell, while in the intracellular space of other cells. Different distributions of nanofibers lead to different protein binding patterns of the nanofibers in two different cell lines. We believe that a useful and versatile platform has been offered to the image cellular distribution of nanofibers, which can provide useful information to the biological functions of the self-assembled nanostructures.


cellular distribution self-assembly peptide nanofiber 


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Supplementary material

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.School of Biomedical EngineeringTianjin Medical UniversityTianjinChina
  2. 2.Tianjin Key Laboratory of Protein ScienceTianjinChina
  3. 3.Department of Plant Biology and Ecology, College of Life SciencesNankai UniversityTianjinChina
  4. 4.Collaborative Innovation Center of Chemical Science and EngineeringTianjinChina
  5. 5.State Key Laboratory of Medicinal Chemical BiologyTianjinChina
  6. 6.College of Life SciencesNankai UniversityTianjinChina

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