Skip to main content
SpringerLink
Log in

Visualization of α1-adrenergic receptors with phenylpiperazine-based fluorescent probes

  • Articles
  • Published:
Science China Chemistry Aims and scope Submit manuscript

Abstract

Several novel fluorescent probes targeting α1-adrenergic receptors were well designed and synthesized by conjugating phenylpiperazine pharmacophore with coumarin and fluorescein fluorophores. These compounds showed suitable fluorescence property, high receptor affinity, and low cytotoxicity. Moreover, the cell imaging results displayed that these probes can be effective tools for the real-time detection of ligand-receptor interactions, as well as the visualization and location of α1-adrenergic receptors in living cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Shi T, Gaivin RJ, McCune DF, Gupta M, Perez DM. J Recept Signal Transduct Res, 2007, 27: 27–45

    Article  CAS  Google Scholar 

  2. Jain KS, Bariwal JB, Kathiravan MK, Phoujdar MS, Sahne RS, Chauhan BS, Shah AK, Yadav MR. Bioorg Med Chem, 2008, 16: 4759–4800

    Article  CAS  Google Scholar 

  3. Ruffolo RR, Hieble JP. Eur Urol, 1999, 36: 17–22

    Article  CAS  Google Scholar 

  4. Kyprianou N, Benning CM. Cancer Res, 2000, 60: 4550–4555

    CAS  Google Scholar 

  5. Sun Q, Li J, Liu WN, Dong QJ, Yang WC, Yang GF. Anal Chem, 2013, 85: 11304–11311

    Article  CAS  Google Scholar 

  6. Wang X, Cui L, Zhou N, Zhu W, Wang R, Qian X, Xu Y. Chem Sci, 2013, 4: 2936–2940

    Article  CAS  Google Scholar 

  7. Liu Z, Wang B, Ma Z, Zhou Y, Du L, Li M. Anal Chem, 2015, 87: 2550–2554

    Article  CAS  Google Scholar 

  8. Chen L, Sun W, Li J, Liu Z, Ma Z, Zhang W, Du L, Xu W, Fang H, Li M. Org Biomol Chem, 2013, 11: 378–382

    Article  CAS  Google Scholar 

  9. Ma Z, Du L, Li M. J Med Chem, 2014, 57: 8187–8203

    Article  CAS  Google Scholar 

  10. Li MY, Fang H, Xia L. Bioorg Med Chem Lett, 2005, 15: 3216–3219

    Article  CAS  Google Scholar 

  11. Du L, Li M. Curr Comput Aided Drug Des, 2010, 6: 165–178

    Article  CAS  Google Scholar 

  12. Li MY, Tsai KC, Xia L. Bioorg Med Chem Lett, 2005, 15: 657–664

    Article  CAS  Google Scholar 

  13. Li M, Xia L. Chem Biol Drug Des, 2007, 70: 461–464

    Article  CAS  Google Scholar 

  14. Zhang W, Ma Z, Li W, Li G, Chen L, Liu Z, Du L, Li M. ACS Med Chem Lett, 2015, 6: 502–506

    Article  CAS  Google Scholar 

  15. Zhang W, Chen L, Ma Z, Du L, Li M. Drug Discov Ther, 2014, 8: 11–17

    Article  CAS  Google Scholar 

  16. Vichai V, Kirtikara K. Nat Protoc, 2006, 1: 1112–1116

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Chemical Biology (Ministry of Education), Shandong University, Jinan, 250012, China

    Yuxing Lin, Wenhua Li, Qinying Yu, Xinyang Zhou, Wei Zhang, Lupei Du & Minyong Li

  2. Department of Medicinal Chemistry, School of Pharmacy, Shandong University, Jinan, 250012, China

    Yuxing Lin, Wenhua Li, Qinying Yu, Xinyang Zhou, Wei Zhang, Lupei Du & Minyong Li

Authors
  1. Yuxing Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenhua Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qinying Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xinyang Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Wei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lupei Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Minyong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Minyong Li.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lin, Y., Li, W., Yu, Q. et al. Visualization of α1-adrenergic receptors with phenylpiperazine-based fluorescent probes. Sci. China Chem. 59, 624–628 (2016). https://doi.org/10.1007/s11426-015-5503-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-015-5503-x

Keywords

Search

Navigation