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Enzyme-free amplified detection of microRNA using target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme

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Abstract

As microRNAs (miRNAs) are aberrantly expressed in a variety of cancers, detecting them precisely is of great importance. Here we constructed a sensitive and selective enzyme-free sensing platform for miRNA detection based on target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme (Mg2+-dependent DNAzyme). This sensing method introduces two amplification circuits simultaneously and shows a low detection limit of 1 pmol/L. This enzyme-free method is especially preferred because of its facility and economy. Furthermore, this amplified sensor shows high selectivity for discriminating perfectly complementary target and other mismatched RNAs. Therefore, the established strategy could be used as a simple, sensitive and selective method for target miRNA detection.

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Authors and Affiliations

  1. Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education; Key Laboratory of Analysis and Detection for Food Safety of Fujian Province; College of Chemistry, Fuzhou University, Fuzhou, 350108, China

    Jinfeng Chen, Bo Liu, Xiaorong Song, Ping Tong, Huanghao Yang & Lan Zhang

  2. Testing Center, the Sport Science Research Center, Fuzhou University, Fuzhou, 350002, China

    Ping Tong & Lan Zhang

  3. Longyan Entry-Exit Inspection and Quarantine Bureau of P.R.C, Longyan, 364000, China

    Jinfeng Chen

  4. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University), Ministry of Education, Yanji, 133002, China

    Huanghao Yang

Authors
  1. Jinfeng Chen
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  2. Bo Liu
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  3. Xiaorong Song
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  4. Ping Tong
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  5. Huanghao Yang
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  6. Lan Zhang
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Correspondence to Ping Tong or Lan Zhang.

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Chen, J., Liu, B., Song, X. et al. Enzyme-free amplified detection of microRNA using target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme. Sci. China Chem. 58, 1906–1911 (2015). https://doi.org/10.1007/s11426-015-5391-0

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  • DOI: https://doi.org/10.1007/s11426-015-5391-0

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