Skip to main content

Advertisement

SpringerLink
Log in

Enhanced dSTORM imaging using fluorophores interacting with cucurbituril

  • Communications
  • SPECIAL TOPIC · New Researches of State Key Laboratories in Analytical Chemistry
  • Published:
Science China Chemistry Aims and scope Submit manuscript

Abstract

Advanced fluorescence microscopy including single-molecule localization-based super-resolution imaging techniques requires bright and photostable dyes or proteins as fluorophores. The photophysical properties of fluorophores have been proven to be crucial for super-resolution microscopy’s localization precision and imaging resolution. Fluorophores TAMRA and Atto Rho6G, which can interact with macrocyclic host cucurbit[7]uril (CB7) to form host-guest compounds, were found to improve the fluorescence intensity and lifetimes of these dyes. We enhanced the localization precision of direct stochastic optical reconstruction microscopy (dSTORM) by introducing CB7 into the imaging buffer, and showed that the number of photons as well as localizations of both TAMRA and Atto Rho6G increase over 2 times.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Betzig E, Patterson GH, Sougrat R. Science, 2006, 313: 1642–1645

    Article  CAS  Google Scholar 

  2. Rust MJ, Bates M, Zhuang XW. Nat Meth, 2006, 3: 793–796

    Article  CAS  Google Scholar 

  3. Heilemann M, van de Linde S, Schüttpelz M, Kasper R, Seefeldt B, Mukherjee A, Tinnefeld P, Sauer M. Angew Chem Int Ed, 2008, 47: 6172–6176

    Article  CAS  Google Scholar 

  4. Schermelleh L, Heintzmann R. J Cell Biol, 2010, 190: 165–175

    Article  CAS  Google Scholar 

  5. Huang B, Bates M, Zhuang XW. Annu Rev Biochem, 2009, 78: 993–1016

    Article  CAS  Google Scholar 

  6. Patterson G, Davidson M, Manley S, Lippincott-Schwartz J. Annu Rev Phys Chem, 2010, 61: 345–367

    Article  CAS  Google Scholar 

  7. Zheng QS, Juette MF, Jockusch S, Wasserman MR, Zhou Z, Altman RB, Blanchard SC. Chem Soc Rev, 2014, 43: 1044–1056

    Article  CAS  Google Scholar 

  8. Mock WL. Cucurbituril. In: Weber E, Ed. Supramolecular Chemistry II-Host Design and Molecular Recognition. Volume 175: Topics in Current Chemistry Berlin Heidelberg: Springer, 1995. 1–24

  9. Hoffmann R, Knoche W, Fenn C, Buschmann HJ. J Chem Soc Faraday Trans, 1994, 90: 1507–1511

    Article  CAS  Google Scholar 

  10. Lee JW, Samal S, Selvapalam N, Kim HJ, Kim K. Accounts Chem Res, 2003, 36: 621–630

    Article  CAS  Google Scholar 

  11. Marquez C, Nau WM. Angew Chem Int Ed, 2001, 40: 4387–4390

    Article  CAS  Google Scholar 

  12. Lagona J, Mukhopadhyay P, Chakrabarti S, Isaacs L. Angew Chem Int Ed, 2005, 44: 4844–4870

    Article  CAS  Google Scholar 

  13. Dsouza RN, Pischel U, Nau WM. Chem Rev, 2011, 111: 7941–7980

    Article  CAS  Google Scholar 

  14. Bhasikuttan AC, Mohanty J, Nau WM, Pal H. Angew Chem Int Ed, 2007, 46: 4120–4122

    Article  CAS  Google Scholar 

  15. Hwang I, Jeon WS, Kim HJ, Kim D, Kim H, Selvapalam N, Fujita N, Shinkai S, Kim K. Angew Chem Int Ed, 2007, 46: 210–213

    Article  CAS  Google Scholar 

  16. Petrov NK, Ivanov DA, Golubkov DV, Gromov SP, Alfimov MV. Chem Phys Lett, 2009, 480: 96–99

    Article  CAS  Google Scholar 

  17. Aliaga ME, Garcia-Rio L, Pessego M, Montecinos R, Fuentealba D, Uribe I, Martin-Pastor M, Garcia-Beltran O. New J Chem, 2015, 39: 3084–3092

    Article  CAS  Google Scholar 

  18. Zhang M, Chen JL, Gao J, Wang ZY, Xu HJ, Cai MJ, Jiang JG, Tian ZY, Wang HD. Phys Chem Chem Phys, 2015, 17: 6722–6727

    Article  CAS  Google Scholar 

  19. Nau WM, Mohanty J. Int J Photoenergy, 2005, 7: 133–141

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry; Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Min Zhang, Jing Gao, Junling Chen, Mingjun Cai, Junguang Jiang & Hongda Wang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Min Zhang, Jing Gao & Junling Chen

  3. School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, 100049, China

    Zhiyuan Tian

Authors
  1. Min Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jing Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Junling Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mingjun Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Junguang Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhiyuan Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hongda Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Zhiyuan Tian or Hongda Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, M., Gao, J., Chen, J. et al. Enhanced dSTORM imaging using fluorophores interacting with cucurbituril. Sci. China Chem. 59, 848–852 (2016). https://doi.org/10.1007/s11426-015-0535-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-015-0535-0

Keywords

Search

Navigation