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Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47

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Abstract

The 4N1K peptide, which is derived from the C-terminal domain of thrombospondin-1 (TSP-1), is usually used as a functional mimic peptide for TSP-1. Knowledge about the interaction force of 4N1K/CD47 is important in explaining how TSP-1 affects the biological effect of CD47. Here we used a single-molecule force spectroscopy (SMFS) technique to explore the interaction of 4N1K/CD47 on both normal and oxidative human red blood cells (hRBCs) at single-molecule level. There was no interaction force between 4N1K and CD47 on normal hRBCs; however, we did find 4N1K-bound CD47 on oxidative hRBCs. We also detected interaction forces for 4N1K/CD47ex (extracellular domain of human CD47), and 4N1K/oxidative CD47ex. The interaction forces of 4N1K/CD47ex were almost consistent with those of 4N1K/oxidative CD47ex at the same loading rate. These results suggest that the conformational change of CD47 is critical for 4N1K-CD47 interaction on oxidative hRBCs.

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    YanGang Pan, Feng Wang & HongDa Wang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    YanGang Pan & HongDa Wang

  3. Institute of Immunology; the First Bethune Hospital Academy of Translational Medicine, Jilin University, Changchun, 130012, China

    YanHou Liu & Yong-Guang Yang

  4. Columbia Center for Translational Immunology, Columbia University, New York, NY, 10032, USA

    Yong-Guang Yang

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  1. YanGang Pan
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  2. Feng Wang
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  5. HongDa Wang
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Correspondence to Yong-Guang Yang or HongDa Wang.

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Pan, Y., Wang, F., Liu, Y. et al. Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47. Sci. China Chem. 57, 1716–1722 (2014). https://doi.org/10.1007/s11426-014-5232-6

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  • DOI: https://doi.org/10.1007/s11426-014-5232-6

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