Abstract
A new method to screen antibiotic combinations is demonstrated, which takes advantage of the logic-signal output of genetically engineered drug-resistant E. coli strains expressing different fluorescent proteins. Thirty-six antibiotic combinations for nine antibiotics were investigated. The operation of different logic gates can reveal the susceptibility, resistance, or synergistic effect of the antibiotic combinations in a rapid (7–8 h versus 24–28 h for typical growth-based assays), simple, quantitative and high-throughput manner. This logic-signal-based output patterns provide the basis for novel and reliable screening of antibiotic combinations and help us to both gain insight into the mechanisms of multi-drug action.
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Lv, F., Liu, L. & Wang, S. Logic-signal output of fluorescent proteins for screening antibiotic combinations. Sci. China Chem. 57, 1696–1702 (2014). https://doi.org/10.1007/s11426-014-5224-6
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DOI: https://doi.org/10.1007/s11426-014-5224-6