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Hemin-histamine-montmorillonite clay conjugate as a model biocatalyst to mimic natural peroxidase

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Abstract

We successfully synthesized the first hemin-montmorillonite bio-conjugate with an amino acid residue to mimic natural peroxidase enzyme. Histamine was intercalated in montmorillonite by cation exchange, then a hemin molecule was loaded onto the histamine-montmorillonite with an adsorption capacity of 7.0 mg g−1. The hemin-histamine-montmorillonite conjugate shows high peroxidase activity as indicated by the oxidation of guaiacol, which is attributed to the activation of hemin by Fe-N complex formation between the imidazole group in histamine and the iron ion in the hemin molecule. Temperature-dependent peroxidase activity for this synthesized biomimetic material indicates that raising the reaction temperature could significantly enhance the activity of the conjugate. The biomimetic catalyst has good reusability; nearly 100% activity can be retained after three cycles. Because montmorillonite clay is widely distributed in the environment, this material offers great potential for in situ and ex situ remediation of many organic contaminants in surface/subsurface soils.

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Authors and Affiliations

  1. State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, China

    LiHui Zhang, Cheng Gu, Jian Xiong, Mei Yang & Yong Guo

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  1. LiHui Zhang
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  2. Cheng Gu
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  3. Jian Xiong
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  4. Mei Yang
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  5. Yong Guo
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Correspondence to Cheng Gu.

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Zhang, L., Gu, C., Xiong, J. et al. Hemin-histamine-montmorillonite clay conjugate as a model biocatalyst to mimic natural peroxidase. Sci. China Chem. 58, 731–737 (2015). https://doi.org/10.1007/s11426-014-5196-6

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