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Photosensitive damage of lysozyme caused by pazufloxacin and the protective effect of ferulic acid

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Abstract

Laser flash photolysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) experiments were carried out to study the photosensitive damage induced by pazufloxacin (PAX) and the protection of lysozyme (Lyso) by ferulic acid (FCA), an active antioxidant. Transient absorption spectra revealed electron transfer from Lyso and FCA to 3PAX*, with respective bimolecular reaction rate constants of 6.3×107 and 1.4×1010 dm3/(mol s). A pulse radiolysis study was also performed to investigate the formation of Lyso radical cations. Results showed that FCA effectively inhibits the cross-linking of protein induced by fluoroquinolones. Finally, a mechanism of the protective effect of FCA on Lyso was proposed.

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Authors and Affiliations

  1. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China

    Yulie Xu, Yancheng Liu, Haixia Li, Peng Zhang, Jianfeng Zhao & Wenfeng Wang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Yulie Xu & Jianfeng Zhao

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  1. Yulie Xu
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  2. Yancheng Liu
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  3. Haixia Li
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  4. Peng Zhang
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  5. Jianfeng Zhao
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  6. Wenfeng Wang
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Correspondence to Wenfeng Wang.

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Xu, Y., Liu, Y., Li, H. et al. Photosensitive damage of lysozyme caused by pazufloxacin and the protective effect of ferulic acid. Sci. China Chem. 58, 508–513 (2015). https://doi.org/10.1007/s11426-014-5174-z

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  • DOI: https://doi.org/10.1007/s11426-014-5174-z

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