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Layer by layer surface engineering of poly (lactide-co-glycolide) nanoparticles: A versatile tool for nanoparticle engineering for targeted drug delivery

Abstract

Recent work regarding the Layer by Layer (LbL) engineering of poly(lactide-co-glycolide) nanoparticles (PLGA NPs) is reviewed here. The LbL engineering of PLGA NPs is applied as a means of generating advanced drug delivery devices with tailored recognition, protection, cargo and release properties. LbL in combination with covalent chemistry is used to attach PEG and folic acid to control cell uptake and direct it towards cancer cells. LbL coatings composed of chitosan and alginate show low protein interactions and can be used as an alternative to Pegylation. The assembly on top of LbL coatings of lipid layers composed of variable percentages of 1,2-dioleoyl-sn-glycero-3-choline (DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) increases NP uptake and directs the NPs towards the endoplasmic reticulum. The antibody anti-TNF-α is encapsulated forming a complex with alginate that is assembled LbL on top of PLGA NPs. The antibody is released in cell culture following first order kinetics. The release kinetics of encapsulated molecules inside PLGA NPs are studied when the PLGA NPs are coated via LbL with different polyelectrolytes. The intracellular release of encapsulated Doxorubicin is studied in the HepG2 cell line by means of Fluorescence Lifetime Imaging.

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Correspondence to Sergio E. Moya.

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Recommended by Prof. GAO Changyou (Zhejiang University)

MOYA Sergio E. (left) studied chemistry at the National University of the South, Argentina and received his Ph.D. in Physical Chemistry from the University of Potsdam, Germany, working at the Max Planck Institute of Colloids and Interfaces. After post doctoral stages at the College de France, Paris, and at the University of Cambridge, UK, he worked for a year and a half at CIQA, Mexico as an independent researcher. After that he joined the Cooperative Centre of Biomaterials in San Sebastian, Spain, as a research group leader. He is also a visiting professor at Zhejiang University, China. His research interests focus on physical chemistry at the nanoscale, soft matter nanotechnology, polyelectrolytes, nanomedicine and nanotoxicology. He is the author of around 75 articles in material science, chemistry, and polymer science.

ROMERO Gabriela (right) received her B.S. in Chemical Engineering from the Autonomous University of San Luis Potosí, Mexico, in 2007. She joined the group of Dr. MOYA at CIC biomaGUNE in 2008 and under her supervision she received a Masters degree in Advanced Materials Engineering from the University of the Basque Country, Spain, in 2009. In the same group she carried out her doctorate studies and she received her Ph.D. degree in Applied Chemistry and Polymer Science from the University of the Basque Country, Spain, in 2012. In October 2012 she joined the group of Dr. Brad Berron as a postdoctoral research associate from the University of Colorado and the University of Kentucky in the USA.

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Romero, G., Murray, R.A., Qiu, Y. et al. Layer by layer surface engineering of poly (lactide-co-glycolide) nanoparticles: A versatile tool for nanoparticle engineering for targeted drug delivery. Sci. China Chem. 56, 1029–1039 (2013). https://doi.org/10.1007/s11426-013-4891-z

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Keywords

  • layer by layer
  • PLGA NPs
  • cell uptake
  • antibody delivery
  • lipid layers
  • intracellular release