Skip to main content
SpringerLink
Log in

Al3+/graphene composites for electrochemical detection of DNA cleavage

  • Articles
  • Published:
Science China Chemistry Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

Herein, we present the electrochemical co-deposition of Al3+/graphene composites directly from an aqueous mixture containing graphene oxide (GO) and Al3+. The obtained Al3+/graphene composites with good electrochemical activity were regarded as an appropriate immobilization platform for double-stranded DNA (dsDNA). The nontoxic redox probe xanthurenic acid (XA) was successfully applied to recognize single-stranded DNA and dsDNA. We illustrated that the scission of dsDNA caused by GO combining with some metal ions could be detected by monitoring the electrochemical signals of XA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Novoselov KS, Geim AK, Morozov SV, Jiang D, Zhang Y, Dubonos SV, Grigorieva IV, Firsov AA. Electric field effect in atomically thin carbon films. Science, 2004, 306:666–669

    Article  CAS  Google Scholar 

  2. Wang T, Zhu Y, Li G, Zhang SY, Song JM, Mao CJ, Wu JY, Jin BK, Tian YP. A novel hydrogen peroxide biosensor based on the BPT/AuNPs/graphene/HRP composite. Sci China Chem, 2011, 54:1645–1650

    Article  CAS  Google Scholar 

  3. Du M, Yang T, Li X, Jiao K. Fabrication of DNA/graphene/polyaniline nanocomplex for label-free voltammetric detection of DNA hybridization. Talanta, 2012, 88:439–444

    Article  CAS  Google Scholar 

  4. Du M, Yang T, Zhao CZ, Jiao K. Electrochemical logic aptasensor based on graphene. Sensor Actuat B Chem, 2012, 169:255–260

    Article  CAS  Google Scholar 

  5. Yang T, Li QH, Li X, Wang XH, Du M, Jiao K. Freely switchable impedimetric detection of target gene sequence based on synergistic effect of ERGNO/PANI nanocomposites. Biosens Bioelectron, 2013, 42:415–418

    Article  CAS  Google Scholar 

  6. Du M, Yang T, Guo XH, Zhong L, Jiao K. Electrochemical synthesis of Fe2O3 on graphene matrix for indicator-free impedimetric aptasensing. Talanta, 2013, 105:229–234

    Article  CAS  Google Scholar 

  7. Yang T, Li X, Li QH, Guo XH, Guan Q, Jiao K. Electrochemically reduced graphene oxide-enhanced electropolymerization of poly-xanthurenic acid for direct, “signal-on” and high sensitive impedimetric sensing of DNA. Polym Chem, 2013, 4:1228–1234

    Article  CAS  Google Scholar 

  8. Liu M, Zhao HM, Chen S, Yu HT, Quan X. Capture of double-stranded DNA in stacked-graphene: giving new insight into the graphene/DNA interaction. Chem Commun, 2012, 48:564–566

    Article  CAS  Google Scholar 

  9. Niu SY, Zhao M, Ren R, Zhang SS. Carbon nanotube-enhanced DNA biosensor for DNA hybridization detection using manganese (II)-Schiff base complex as hybridization indicator. J Inorg Biochem, 2009, 103:43–49

    Article  CAS  Google Scholar 

  10. Erdem A, Kerman K, Meric B, Ozsoz M. Methylene blue as a novel electrochemical hybridization indicator. Electroanal, 2001, 13:219–223

    Article  CAS  Google Scholar 

  11. Sturney RG, Wild CP, Hardie LJ. Removal of red light minimizes methylene blue-stimulated DNA damage in oesophageal cells: implications for chromoendoscopy. Mutagenesis, 2009, 24:253–258

    Article  Google Scholar 

  12. Shen L, Ji HF. Theoretical exploration of the photosensitive properties of xanthurenic acid, a tryptophan metabolite in cataractous human lenses. Dyes Pigments, 2008, 76:646–649

    Article  CAS  Google Scholar 

  13. Zhang W, Yang T, Jiao K. Ultrasensitive indicator-free and enhanced self-signal nanohybrid DNA sensing platform based on electrochemically grown poly-xanthurenic acid/Fe2O3 membranes. Biosens Bioelectron, 2013, 31:182–189

    Article  Google Scholar 

  14. Kovtyukhova NI, Ollivier PJ, Martin BR, Mallouk TE, Chizhik SA, Buzaneva EV, Gorchinskiy AD. Layer-by-layer assembly of ultrathin composite films from micron-sized graphite oxide sheets and polycations. Chem Mater, 1999, 11:771–778

    Article  CAS  Google Scholar 

  15. Wang G, Yang J, Park J, Gou X, Wang B, Liu H, Yao J. Facile synthesis and characterization of graphene nanosheets. J Phys Chem C, 2008, 112:8192–8195

    Article  CAS  Google Scholar 

  16. Duskova K, Sierra S, Fernandez MJ, Gude L, Lorente A. Synthesis and DNA interaction of ethylenediamine platinum(II) complexes linked to DNA intercalants. Bioorgan Med Chem, 2012, 20:7112–7118

    Article  CAS  Google Scholar 

  17. Wong EL, Gooding JJ. Electronic detection of target nucleic acids by a 2,6-disulfonic acid anthraquinone intercalator. Anal Chem, 2003, 75:3845–3852

    Article  CAS  Google Scholar 

  18. Wong EL, Gooding JJ. Charge transfer through DNA: A selective electrochemical DNA biosensor. Anal Chem, 2006, 78:2138–2144

    Article  CAS  Google Scholar 

  19. Wang ZJ, Zhang J, Yin ZY, Wu SX, Mandler D, Zhang H. Fabrication of nanoelectrode ensembles by electrodepositon of Au nanoparticles on single-layer graphene oxide sheets. Nanoscale, 2012, 4:2728–2733

    Article  CAS  Google Scholar 

  20. Mancin F, Scrimin P, Tecilla P, Tonellato U. Artificial metallonucleases. Chem Commun, 2005, 2540–2548

    Google Scholar 

  21. Biver T, Secco F, Venturini M. Mechanistic aspects of the interaction of intercalating metal complexes with nucleic acids. Coord Chem Rev, 2008, 252:1163–1177

    Article  CAS  Google Scholar 

  22. Friedman AE, Chambron JC, Sauvage JP, Turro NJ, Barton JK. Molecular light switch for DNA: Ru(bpy)2(dppz)2+. J Am Chem Soc, 1990, 112:4960–4962

    Article  CAS  Google Scholar 

  23. Sheng X, Lu XM, Chen YT, Lu GY, Zhang JJ, Shao Y, Liu F, Xu Q. Synthesis, DNA-binding, cleavage, and cytotoxic activity of new 1,7-dioxa-4,10-diazacyclododecane artificial receptors containing bisguanidinoethyl or diaminoethyl double side arms. Chem Eur J, 2007, 13:9703–9712

    Article  CAS  Google Scholar 

  24. Camargo MA, Neves A, Bortoluzzi AJ, Szpoganicz B, Fischer FL, Terenzi H, Serra OA, Santos VG, Vaz BG, Eberlin MN. Efficient phosphodiester hydrolysis by luminescent terbium (III) and europium (III) Complexes. Inorg Chem, 2010, 49:6013–6025

    Article  CAS  Google Scholar 

  25. Pitie M, Pratviel GV. Activation of DNA carbon-hydrogen bonds by metal complexes. Chem Rev, 2010, 110:1018–1059

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Meng Du, QianQian Kong, Tao Yang & Kui Jiao

Authors
  1. Meng Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. QianQian Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tao Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kui Jiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kui Jiao.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Cite this article

Du, M., Kong, Q., Yang, T. et al. Al3+/graphene composites for electrochemical detection of DNA cleavage. Sci. China Chem. 56, 1325–1330 (2013). https://doi.org/10.1007/s11426-013-4858-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-013-4858-0

Keywords

Search

Navigation