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Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia

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Abstract

The objective of the present study is to develop a method for large-scale separating and purifying salidroside from rhodiola kirilowii roots and for preparing injectable medicinal ingredient. Crude extract of salidroside was prepared by water-ethanol system, and purified by column chromatography of macroporous resins. Static adsorption and desorption studies were performed on six kinds of macroporous resins, and SP825 resin was chosen, followed by optimizing process parameters. The optimum sample volume, feed concentration, ratio of diameter to height, and feeding flow rate were 1.5 bed volumes (BV), 15 mg/mL, 1:10 and 1 BV/h, respectively. Dynamic desorption was performed consecutively with 8 BV of distilled water, 3 BV of 5% ethanol and 8 BV of 10% ethanol at a flow rate of 2 BV/h. After three cycles in separating 3.5 tons of rhodiola kirilowii roots, salidroside purity was increased from 3.4% in the crude extract to 93.6% in purified salidroside product. This study provides a novel method to separate salidroside for injectable use.

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Authors and Affiliations

  1. State Key Laboratory of Natural and Biomimetic Drugs; School of Pharmaceutical Sciences, Peking University, Beijing, 100191, China

    RuiJun Ju, Jia Zhou, RuoJing Li, Peng Zhou, Qiang Zhang & WanLiang Lu

  2. Department of Pharmacy, Fujian Health College, Fuzhou, 350101, China

    RenJie Huang

  3. China Academy of Chinese Medical Sciences, Beijing, 100700, China

    ZaoHua Zhang

  4. Kangmei Pharmaceutical Co., Ltd., Puning, 515300, China

    FeiJun Xiang, DongJin Xu, WeiXiang Liu & XingTian Ma

Authors
  1. RuiJun Ju
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  2. RenJie Huang
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  3. Jia Zhou
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  4. RuoJing Li
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  5. Peng Zhou
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  6. ZaoHua Zhang
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  7. FeiJun Xiang
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  8. DongJin Xu
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  9. WeiXiang Liu
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  10. XingTian Ma
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  11. Qiang Zhang
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  12. WanLiang Lu
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Correspondence to WanLiang Lu.

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Contributed equally to this work.

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Ju, R., Huang, R., Zhou, J. et al. Separation of injectable salidroside by column chromatography of macroporous resins for treating myocardial ischemia. Sci. China Chem. 55, 1435–1444 (2012). https://doi.org/10.1007/s11426-011-4471-z

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  • DOI: https://doi.org/10.1007/s11426-011-4471-z

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