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Electrochemiluminescence glucose biosensor based on glucose dehydrogenase functionalized Ru(bpy)3 2+ doped silica nanoparticles

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Abstract

A novel electrochemiluminescence (ECL) sensing approach was developed for glucose detection based on crosslinking Ru(bpy)3Cl2-doped silica nanoparticles (RuSiNPs) with glucose dehydrogenase on a glassy carbon electrode (GCE). Glutaraldehyde and aminopropyltrimethoxysilane were used as linking agents, and chitosan was used to immobilize the composites onto the GCE surface. The ECL sensor presented good characteristics in terms of stability and reproducibility. Under optimized conditions, the linear response of ECL intensity to glucose concentration was valid in the range of 0.2 to 20 mmol/L (R 2 = 0.9962). The application results indicated that the proposed approach is with great potential in the determination of glucose.

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Authors and Affiliations

  1. College of Sciences, Hebei University of Science and Technology, Shijiazhuang, 050018, China

    GuangMei Guo

  2. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China

    Xi Chen

  3. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    QingAi Chen

Authors
  1. GuangMei Guo
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  2. QingAi Chen
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  3. Xi Chen
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Correspondence to Xi Chen.

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Guo, G., Chen, Q. & Chen, X. Electrochemiluminescence glucose biosensor based on glucose dehydrogenase functionalized Ru(bpy)3 2+ doped silica nanoparticles. Sci. China Chem. 54, 1777–1781 (2011). https://doi.org/10.1007/s11426-011-4389-5

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  • DOI: https://doi.org/10.1007/s11426-011-4389-5

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