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Oxidation and detection of L-cysteine using a modified Au/Nafion/glass carbon electrode

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Abstract

In this work, the electrochemical oxidation of L-cysteine (CySH) was investigated on a composite film modified electrode with Au nanoparticles dispersed in the fluorocarbon polymer (Nafion). The excellent electrocatalytic effect on CySH oxidation was attributed to the role of Au nanoparticles. The voltammetric studies revealed two anodic peaks for the oxidation of CySH in the pH range of 2.0–8.0. The electrode was used to detect cysteine at pH 2.0 and pH 7.0. At pH 2.0, a determination range of 3.0–50.0 μmol/L was obtained with the detection sensitivity of 22.7 μA/(mmol L−1), while at pH 7.0, a determination range of 2.0–80.0 μmol/L was obtained with the detection sensitivity of 4.08 μA/(mmol L−1). The detection limits were estimated to be as low as 1.0 μmol/L at both pH 7.0 and pH 2.0. Additionally, at pH 7.0, the interferences of ascorbic acid and uric acid were reduced for the detection of cysteine. These made the Au/Nafion/GC electrode a promising candidate for efficient electrochemical sensors for the detection of CySH.

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References

  1. Banina FG, Moreira JC, Fogg AG. Application of catalytic stripping voltammetry for the determination of organic sulfur-compounds at a hanging mercury drop electrode — behavior of cysteine, cystine and N-acetylcysteine in the presence of nickel ion. The Analyst, 1994, 119: 309–318

    Article  Google Scholar 

  2. Koryta J, Pradáč J. Electrode processes of the sulfhydryl-disulfide system: III. Cysteine at platinum and gold electrodes. J Electroanal Chem, 1968, 17: 185–189

    Article  CAS  Google Scholar 

  3. Fawcett WR, Fedurco M, Kovacova Z, Borkowska Z. Oxidation of cysteine, cysteinesulfinic acid and cysteic acid on a polycrystalline gold electrode. J Electroanal Chem, 1994, 368: 265–274

    Article  CAS  Google Scholar 

  4. Reynaud JA, Maltoy B, Canessan P. Electrochemical investigations of amino acids at solid electrodes. Part I. Sulfur components: Cystine, cysteine, methionine. J Electroanal Chem, 1980, 114: 195–211

    Article  CAS  Google Scholar 

  5. Johll ME, Williams DG, Johnson DC. Activated pulsed amperometric detection of cysteine at platinum electrodes in acidic media. Electroanal, 1997, 9: 1397–1402

    Article  CAS  Google Scholar 

  6. Lock J, Davis J. The determination of disulphide species within physiological fluids. Trac-trend Anal Chem, 2002, 21: 807–815

    Article  CAS  Google Scholar 

  7. White PC, Lawrence NS, Davis J, Compton RG. Electrochemical determination of thiols: A perspective. Electroanal, 2002, 14: 89–98

    Article  CAS  Google Scholar 

  8. Tan WT, Bond AM, Ngooi SW, Lim EB, Goh JK. Electrochemical oxidation of L-cysteine mediated by a fullerene-C-60-modified carbon electrode. Anal Chim Acta, 2003, 491: 181–191

    Article  CAS  Google Scholar 

  9. Spãtaru N, Sarada BV, Popa E, Tryk DA, Fujishima A. Voltammetric determination of L-cysteine at conductive diamond electrodes. Anal Chem, 2001, 73: 514–519

    Article  CAS  Google Scholar 

  10. Gong KP, Zhu XZ, Zhao R, Xiong SX, Mao LQ, Chen CF. Rational attachment of synthetic triptycene orthoquinone onto carbon nanotubes for electrocatalysis and sensitive detection of thiols. Anal Chem, 2005, 77: 8158–8165

    Article  CAS  Google Scholar 

  11. Zhao YD, Zhang WD, Chen H, Luo QM. Electrocatalytic oxidation of cysteine at carbon nanotube powder microelectrode and its detection. Sensor Actuat B-Chem, 2003, 92: 279–285

    Article  CAS  Google Scholar 

  12. Zhou M, Ding J, Guo LP, Shang QK. Electrochemical behavior of l-cysteine and its detection at ordered mesoporous carbon-modified glassy carbon electrode. Anal Chem, 2007, 79: 5328–5335

    Article  CAS  Google Scholar 

  13. Hu K, Lan D, Li X, Zhang S. Electrochemical DNA biosensor based on nanoporous gold electrode and multifunctional encoded DNA-Au Bio Bar codes. Anal Chem, 2008, 80: 9124–9130

    Article  CAS  Google Scholar 

  14. Majid E, Hrapovic S, Liu YL, Male KB, Luong JHT. Electrochemical determination of arsenite using a gold nanoparticle modified glassy carbon electrode and flow analysis. Anal Chem, 2006, 78: 762–769

    Article  CAS  Google Scholar 

  15. Xiang C, Zou Y, Sun LX, Xu F. Direct electrochemistry and enhanced electrocatalysis of horseradish peroxidase based on flowerlike ZnO-gold nanoparticle-Nafion nanocomposite. Sensor Actuat B-Chem, 2009, 136: 158–162

    Article  CAS  Google Scholar 

  16. Thangavel S, Ramaraj R. Polymer membrane stabilized gold nanostructures modified electrode and its application in nitric oxide detection. J Phys Chem C, 2008, 112: 19825–19830

    Article  CAS  Google Scholar 

  17. Li J, Lin XQ. Electrodeposition of gold nanoclusters on overoxidized polypyrrole film modified glassy carbon electrode and its application for the simultaneous determination of epinephrine and uric acid under coexistence of ascorbic acid. Anal Chim Acta, 2007, 596: 222–230

    Article  CAS  Google Scholar 

  18. Cai WY, Xu Q, Zhao XN, Zhu JH, Chen HY. Porous gold-nanoparticle-CaCO3 hybrid material: Preparation, characterization, and application for horseradish peroxidase assembly and direct electrochemistry. Chem Mater, 2006, 18: 279–284

    Article  CAS  Google Scholar 

  19. Kong LL, Kan MX, Han DM, Wang T, Zhang HM. Highly sensitive response to dopamine at a modified electrode involving a composite film with Au nanoparticles dispersed in a fluorocarbon polymer. Sci China Chem, 2010, 53: 826–831

    Article  CAS  Google Scholar 

  20. Zen JM, Kumar AS, Chen JC. Electrocatalytic oxidation and sensitive detection of cysteine on a lead ruthenate pyrochlore modified electrode. Anal Chem, 2001, 73: 1169–1175

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Science, Beijing Institute of Technology, Beijing, 100081, China

    XueJi Wang, LiLi Zhang, LiXiao Miao, MeiXiu Kan, LinLin Kong & HuiMin Zhang

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  1. XueJi Wang
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  2. LiLi Zhang
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  3. LiXiao Miao
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  5. LinLin Kong
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Correspondence to HuiMin Zhang.

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Wang, X., Zhang, L., Miao, L. et al. Oxidation and detection of L-cysteine using a modified Au/Nafion/glass carbon electrode. Sci. China Chem. 54, 521–525 (2011). https://doi.org/10.1007/s11426-011-4233-y

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  • DOI: https://doi.org/10.1007/s11426-011-4233-y

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