Skip to main content
SpringerLink
Log in

In situ evaluation of cell cultivation on dynamically changed poly(ɛ-caprolactone) film caused by enzymatic degradation

  • Articles
  • Special Issue
  • Published:
Science China Chemistry Aims and scope Submit manuscript

Abstract

In situ evaluation of cell cultivation on degrading poly(ɛ-caprolactone) (PCL) films was studied. New culture surroundings were constructed for cell growth by using PCL films as substrates and adding Pseudomonas cepacia lipase to accelerate biodegradation of PCL films. MTT experiments for 10 h indicated the low cytotoxicity of lipase solution with concentration up to 0.2 mg/mL for MG-63 cells growth on PCL films. With the optimized lipase concentration and degradation time, we studied cell growth behavior on dynamically changed PCL films by adding lipase to the culture surroundings. MTT, fluorescence microscopy and scanning electron microscopy (SEM) were used to evaluate cell viability, proliferation and morphologies. It was found that cell viability and proliferation were not affected by the added lipase solution negatively. In contrast, cells cultured on degrading PCL films showed good growth behavior with clear fusiform shape and pseudopods. Importantly, the enzymatic degradation of PCL films with cells attachment showed distinctive morphology compared to the degradation in lipase solution without cells. The simultaneous cell growth and PCL film degradation were well discussed in this work, which may better understand the interaction between cell growth and polymer degradation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Zhang H, Bei J, Wang S. Multi-morphological biodegradable PLGE nanoparticles and their drug release behavior. Biomaterials, 2009, 30: 100–107

    Article  CAS  Google Scholar 

  2. Zolnik BS, Burgess DJ. Effect of acidic pH on PLGA microsphere degradation and release. J Control Rel, 2007, 122: 338–344

    Article  CAS  Google Scholar 

  3. Chen Y, Chen N, Qiu Z, Wang L, Wan C, Luo X, Li S. Behavior of endothelial cells regulated by a dynamically changed microenvironment of biodegradable PLLA-PC. Macromol Biosci, 2009, 9: 413–420

    Article  CAS  Google Scholar 

  4. Chen X, Ooi CP. Hydrolytic degradation and drug release properties of ganciclovir-loaded biodegradable microspheres. Acta Biomaterialia, 2008, 4: 1046–1056

    Article  CAS  Google Scholar 

  5. Sivak WN, Pollack IF, Petoud S, Zamboni WC, Zhang J, Beckman EJ. LDI-glycerol polyurethane implants exhibit controlled release of DB-67 and anti-tumor activity in vitro against malignant gliomas. Acta Biomaterialia, 2008, 4: 852–862

    Article  CAS  Google Scholar 

  6. Hunziker EB. Articular cartilage repair: Basic science and clinical progress. A review of the current status and prospects. Osteoarthritis and Cartilage, 2002, 10: 432–463

    CAS  Google Scholar 

  7. Sung H-J, Meredith C, Johnson C, Galis ZS. The effect of scaffold degradation rate on three-dimensional cell growth and angiogenesis. Biomaterials, 2004, 25: 5735–5742

    Article  CAS  Google Scholar 

  8. Lee SJ, Khang G, Lee HB. Interaction of fibroblasts on polycarbonate membrane surfaces with different micropore sizes and hydrophilicity. J Biomaterials Sci, Polym Ed, 1999, 10: 283–294

    Article  CAS  Google Scholar 

  9. Lee JH, Lee SJ, Khang G, Lee HB. The effect of fluid shear stress on endothelial cell adhesiveness to polymer surfaces with wettability gradient. J Colloid Interf Sci, 2000, 230: 84–90

    Article  CAS  Google Scholar 

  10. Lee SJ, Gilson K, Lee, YM, Lee HB. Interaction of human chondrocytes and NIH/3T3 fibroblasts on chloric acid-treated biodegradable polymer surfaces. J Biomater Sci, Polym Ed, 2002, 13: 197–212

    Article  CAS  Google Scholar 

  11. Lee SJ, Khang G, Lee YM, Lee HB. The effect of surface wettability on induction and growth of neurites from the PC-12 cell on a polymer surface. J Colloid Interf Sci, 2003, 259: 228–235

    Article  CAS  Google Scholar 

  12. Lee SJ, Choi JS, Park KS, Khang G, Lee YM, Lee HB. Response of MG63 osteoblast-like cells onto polycarbonate membrane surfaces with different micropore sizes. Biomaterials, 2004, 25: 4699–4707

    Article  CAS  Google Scholar 

  13. Maroudas NG. Sulphonated polystyrene as an optimal substratum for the adhesion and spreading of mesenchymal cells in monovalent and divalent saline solutions. J Cell Physiol, 1977, 90: 511–520

    Article  CAS  Google Scholar 

  14. Schakenraad JM, Busscher HJ, Wildevuur CRH. The influence of substratum surface free energy on growth and spreading of human fibroblasts in the presence and absence of serum proteins. J Biomed Mater Res, 1986, 20: 773–784

    Article  CAS  Google Scholar 

  15. Maroudas NG. On the low adhesiveness of fluid phospholipid substrata. J Theor Biol, 1979, 79: 101–116

    Article  CAS  Google Scholar 

  16. Boyan BD, Hummert TW, Dean DD, Schwartz Z. Role of material surfaces in regulating bone and cartilage cell response. Biomaterials, 1996, 17: 137–146

    Article  CAS  Google Scholar 

  17. Brunette DM. The effects of implant surface topography on the behavior of cells. Int. J Oral Maxillofac Implants, 1988, 3: 231–246

    CAS  Google Scholar 

  18. Cheng Z, Teoh S-H. Surface modification of ultra thin poly(ɛ-caprolactone) films using acrylic acid and collagen. Biomaterials, 2004, 25: 1991–2001

    Article  CAS  Google Scholar 

  19. Wei GB, Ma PX. Macroporous and nanofibrous polymer scaffolds and polymer/bone-like apatite composite scaffolds generated by sugar spheres. J Biomed Mater Res Part A, 2006, 78A: 306–315

    Article  CAS  Google Scholar 

  20. Hatano K, Inoue H, Kojo T, Matsunaga T, Tsujisawa T, Uchiyama C, Uchida Y. Effect of surface roughness on proliferation and alkaline phosphatase expression of rat calvarial cells cultured on polystyrene. Bone, 1999, 25: 439–445

    Article  CAS  Google Scholar 

  21. Peng M, Liu W, Yang G, Chen Q, Luo S, Zhao G, Yu L. Investigation of the degradation mechanism of cross-linked polyethyleneimine by NMR spectroscopy. Polym Degrad Stab, 2008, 93: 476–482

    Article  CAS  Google Scholar 

  22. Qian Z, Li S, He Y, Zhang H, Liu X. Hydrolytic degradation study of biodegradable polyesteramide copolymers based on ɛ-caprolactone and 11-aminoundecanoic acid. Biomaterials, 2004, 25: 1975–1981

    Article  CAS  Google Scholar 

  23. Tsuji H, Ono T, Saeki T, Daimon H, Fujie K. Hydrolytic degradation of poly(ɛ-caprolactone) in the melt. Polym Degrad Stab, 2005, 89: 336–343

    Article  CAS  Google Scholar 

  24. Zhang Y, Sun L, Jiang J, Zhang XL, Ding WJ, Gan ZH. Biodegradation-induced surface change of polymer microspheres and its influence on cell growth. Polym Degrad Stab, 2010, 95: 1356–1364

    Article  CAS  Google Scholar 

  25. Choi JS, Lee SJ, Christ GJ, Atala A, Yoo JJ. The influence of electrospun aligned poly(ɛ-caprolactone)/collagen nanofiber meshes on the formation of self-aligned skeletal muscle myotubes. Biomaterials, 2008, 29: 2899–2906

    Article  CAS  Google Scholar 

  26. Calil MR, Gaboardi F, Bardi MAG, Rezende ML, Rosa DS. Enzymatic degradation of poly(ɛ-caprolactone) and cellulose acetate blends by lipase and α-amylase. Polym Testing, 2007, 26: 257–261

    Article  CAS  Google Scholar 

  27. Chong EJ, Phan TT, Lim IJ, Zhang YZ, Bay BH, Ramakrishna S, Lim CT. Evaluation of electrospun PCL/gelatin nanofibrous scaffold for wound healing and layered dermal reconstitution. Acta Biomaterialia, 2007, 3: 321–330

    Article  CAS  Google Scholar 

  28. Chum ZZ, Woodruff MA, Cool SM, Hutmacher DW. Porcine bone marrow stromal cell differentiation on heparin-adsorbed poly(ɛ-caprolactone)-tricalcium phosphate-collagen scaffolds. Acta Biomaterialia, 2009, 5: 3305–3315

    Article  CAS  Google Scholar 

  29. Wiria FE, Leong KF, Chua CK, Liu Y. Poly(ɛ-caprolactone)/hydroxyapatite for tissue engineering scaffold fabrication via selective laser sintering. Acta Biomaterialia, 2007, 3: 1–12

    Article  CAS  Google Scholar 

  30. Yu GQ, Zhang Y, Shi XD, Li ZS, Gan ZH. Surface property and in vitro biodegradation of microspheres fabricated by poly(ɛ-caprolactone-b-ethylene oxide) diblock copolymers. J Biomed Mater Res Part A, 2008, 84A: 926–939

    Article  CAS  Google Scholar 

  31. Zhou S, Deng X, Yang H. Biodegradable poly(ɛ-caprolactone)-poly (ethylene glycol) block copolymers: Characterization and their use as drug carriers for a controlled delivery system. Biomaterials, 2003, 24: 3563–3570

    Article  CAS  Google Scholar 

  32. Sun H, Mei L, Song C, Cui X, Wang P. The in vivo degradation, absorption and excretion of PCL-based implant. Biomaterials, 2006, 27: 1735–1740

    Article  CAS  Google Scholar 

  33. Yim EKF, Wen J, Leong KW. Enhanced extracellular matrix production and differentiation of human embryonic germ cell derivatives in biodegradable poly(ɛ-caprolactone-co-ethyl ethylene phosphate) scaffold. Acta Biomaterialia, 2006, 2: 365–376

    Article  Google Scholar 

  34. Gan Z, Liang Q, Zhang J, Jing X. Enzymatic degradation of poly(ɛ-caprolactone) film in phosphate buffer solution containing lipases. Polym Degrad Stab, 1997, 56: 209–213

    Article  CAS  Google Scholar 

  35. Gan Z, Yu D, Zhong Z, Liang Q, Jing X. Enzymatic degradation of poly(ɛ-caprolactone)/poly(DL-lactide) blends in phosphate buffer solution. Polymer, 1999, 40: 2859–2862

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Engineering Plastics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China

    Yu Zhang, Ni Jiang & ZhiHua Gan

Authors
  1. Yu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ni Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. ZhiHua Gan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to ZhiHua Gan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, Y., Jiang, N. & Gan, Z. In situ evaluation of cell cultivation on dynamically changed poly(ɛ-caprolactone) film caused by enzymatic degradation. Sci. China Chem. 54, 369–374 (2011). https://doi.org/10.1007/s11426-010-4214-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-010-4214-6

Keywords

Search

Navigation