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Nanotexture optimization by oxygen plasma of mesoporous silica thin film for enrichment of low molecular weight peptides captured from human serum

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  • Special Topic · Cancer Nanotechnology
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Abstract

The optimization of mesoporous silica thin films by nanotexturing using oxygen plasma versus thermal oxidation was investigated. Calcination in oxygen plasma provides superior control over pore formation with regard to the pore surface and higher fidelity to the structure of the polymer template. The resulting porous film offers an ideal substrate for the selective partitioning of peptides from complex mixtures. The improved chemico-physical characteristics of porous thin films (pore size distribution, nanostructure, surface properties and pore connectivity) were systematically characterized with XRD, Ellipsometry, FTIR, TEM and N2 adsorption/desorption isotherm. The enrichment of low molecular weight proteins captured from human serum on mesoporous silica thin films fabricated by both methodologies was investigated by comparison of their MALDI-TOF MS profiles. This novel on-chip fractionation technology offers advantages in recovering the low molecular weight peptides from human serum, which has been recognized as an informative resource for early diagnosis of cancer and other diseases.

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Authors and Affiliations

  1. Department of Nanomedicine and Biomedical Engineering, The University of Texas Health Science Center at Houston, Houston, 77031, USA

    Ye Hu, Yang Peng, Louis Brousseau, Ali Bouamrani, XueWu Liu & Mauro Ferrari

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  1. Ye Hu
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  2. Yang Peng
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  3. Louis Brousseau
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  4. Ali Bouamrani
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  5. XueWu Liu
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  6. Mauro Ferrari
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Correspondence to Mauro Ferrari.

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Hu, Y., Peng, Y., Brousseau, L. et al. Nanotexture optimization by oxygen plasma of mesoporous silica thin film for enrichment of low molecular weight peptides captured from human serum. Sci. China Chem. 53, 2257–2264 (2010). https://doi.org/10.1007/s11426-010-4121-x

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  • DOI: https://doi.org/10.1007/s11426-010-4121-x

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