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A whisker-like carbon composite for the immobilization of laccase and its bioelectrochemistry

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Abstract

A novel mesoporous carbon/whisker-like carbon (MCWC) composite was used for the immobilization of laccase (Lac) and its bioelectrochemical behaviors were studied. It was confirmed by XPS that Lac was strongly adsorbed on the surface of the MCWC composite. The cyclic voltammetric results showed that the immobilized Lac underwent a direct quasi-reversible electrochemical reaction. The value of the electron transfer rate constant k s was estimated to be 0.770 s−1, indicating a reasonably fast electron transfer between the immobilized Lac and the underlying electrode. The surface concentration (Γ) of Lac was estimated to be 2.730 × 10−12 mol/cm2. Further experimental results showed that the immobilized Lac displayed an appreciable electrocatalytic activity to the electrochemical reduction of O2. These properties could be attributed to the particular structure of loosely packed nanometer-scale carbon whiskers and the existence of a large amount of oxygen-containing groups. The immobilization method and the novel carrier (MCWC) may find new applications in fabricating the biocatalysts for biofuel cells.

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Authors and Affiliations

  1. Department of Chemical Engineering, Shanghai Institute of Technology, Shanghai, 200233, China

    GuoXian Ma

  2. School of Materials Science and Engineering, Shanghai University, Shanghai, 200072, China

    GuoXian Ma & QingDong Zhong

  3. Department of Chemistry, Nanjing Normal University, Nanjing, 210097, China

    TianHong Lu

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  1. GuoXian Ma
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  2. QingDong Zhong
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  3. TianHong Lu
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Correspondence to GuoXian Ma.

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Ma, G., Zhong, Q. & Lu, T. A whisker-like carbon composite for the immobilization of laccase and its bioelectrochemistry. Sci. China Chem. 53, 1332–1336 (2010). https://doi.org/10.1007/s11426-010-3186-x

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  • DOI: https://doi.org/10.1007/s11426-010-3186-x

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