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Extended detection range for an optical enzymatic glucose sensor coupling with a novel data-processing method

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Abstract

A new data-processing method was established and applied for optical enzymatic glucose sensing, in which oxygen and glucose were simultaneously consumed. The oxygen level remaining in the detection system, which was equal to the difference between the initial and consumed oxygen concentrations, could be measured using fluorescent oxygen indicators immobilized in the sensing layer. It was deduced that the ratio of I 0 and I was inversely proportional to glucose concentration, where I 0 is the maximum fluorescence intensity in various glucose solutions, and I is the fluorescence intensity at various concentrations of glucose. Using the new data-processing method, the detection range of the calibration curve method was extended from 0 to 1.2 mmol L−1, which was enlarged about 2–3 folds over that in ordinary approaches. The prepared glucose sensor could be directly applied to detect high concentrations of glucose.

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Authors and Affiliations

  1. College of Sciences, Hebei University of Science and Technology, Shijiazhuang, 050018, China

    GuangMei Guo

  2. Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University; Key Laboratory of Analytical Sciences of Xiamen University, Xiamen, 361005, China

    XuDong Wang, TingYao Zhou & Xi Chen

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  1. GuangMei Guo
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  2. XuDong Wang
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  3. TingYao Zhou
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  4. Xi Chen
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Correspondence to Xi Chen.

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Guo, G., Wang, X., Zhou, T. et al. Extended detection range for an optical enzymatic glucose sensor coupling with a novel data-processing method. Sci. China Chem. 53, 1385–1390 (2010). https://doi.org/10.1007/s11426-010-3161-6

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  • DOI: https://doi.org/10.1007/s11426-010-3161-6

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