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Fabrication of the DNA/poly(3-methylthiophene) composite film modified electrode and its application for the study on the voltammetric behavior and determination of 8-hydroxy-2′-deoxyguanosine

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Abstract

A composite film of DNA/poly(3-methylthiophene) (P3MT) modified glassy carbon electrode (GCE) has been fabricated by electro-deposition method. P3MT film was first electropolymerized at the GCE and the DNA layer was then immobilized on the P3MT layer by electrochemical method. The voltammetric behavior of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) at the composite film modified electrode was studied. The effects of scan rates, pH and the interference of uric acid (UA) on the voltammetric behavior and detection of 8-OH-dG were also discussed. The experimental results suggest that the electrochemical behavior of 8-OH-dG at the composite film modified electrode was greatly improved due to the combination of the advantages of P3MT and DNA. In 0.1 M pH 7.0 phosphate buffer solution (PBS), the anodic peak currents of 8-OH-dG were linear with the 8-OH-dG concentration in two intervals, viz. 0.28–4.2 µM and 4.2–19.6 µM. The detection limit of 56 nM 8-OH-dG could be estimated (S/N = 3). This proposed composite film modified electrode shows excellent reproducibility and stability. It may have the potential application for the detection of 8-OH-dG in human urine.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Institute of Analytical Chemistry, Liaocheng University, Liaocheng, 252059, China

    YanHuai Wang, Jing Li, Yan Liu, RongNa Ma, WenLi Jia, Hui Cui & HuaiSheng Wang

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  1. YanHuai Wang
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  2. Jing Li
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  3. Yan Liu
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Correspondence to HuaiSheng Wang.

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Supported by the National Natural Science Foundation of China (Grant Nos. 20475024 & 20775031) and the Shandong Tai-Shan Scholar Research Fund

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Wang, Y., Li, J., Liu, Y. et al. Fabrication of the DNA/poly(3-methylthiophene) composite film modified electrode and its application for the study on the voltammetric behavior and determination of 8-hydroxy-2′-deoxyguanosine. Sci. China Ser. B-Chem. 52, 2006–2012 (2009). https://doi.org/10.1007/s11426-009-0241-6

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