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Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

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Abstract

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in micro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5–250 ng·mL−1. The detection limit of CEA was 0.61 ng·mL−1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. Compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

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Authors and Affiliations

  1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

    ZhiYong Li & LiXia Zhao

  2. Department of Chemistry, Tsinghua University, Beijing, 100084, China

    QianYun Zhang & JinMing Lin

  3. Beijing Chemclin Biotech Co., Ltd., Beijing Academy of Science and Technology, Beijing, 100094, China

    ZhenJia Li & GuoMao Hu

  4. School of Chemistry and Chemical Engineering of Xianyang Normal College, Xianyang, 712000, China

    Shan Wang

Authors
  1. ZhiYong Li
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  2. QianYun Zhang
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  3. LiXia Zhao
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  4. ZhenJia Li
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  5. GuoMao Hu
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  6. JinMing Lin
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  7. Shan Wang
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Corresponding authors

Correspondence to LiXia Zhao or JinMing Lin.

Additional information

Support from the CAS Major Scientific Research and Equipment Development Projects (YZ-0632), the National Natural Science Founolation of China (Grant No. 20907060), the CAS Graduate Innovation Foundation (No. YXLW-3), the National key Technology R&D Program (Grant No. 2006BAF07B03-1-1) and Shaanxi Province, China’s Scholarship Council (Grant No. 08JK482)

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Li, Z., Zhang, Q., Zhao, L. et al. Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis. Sci. China Chem. 53, 812–819 (2010). https://doi.org/10.1007/s11426-009-0168-y

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  • DOI: https://doi.org/10.1007/s11426-009-0168-y

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