Skip to main content
SpringerLink
Log in

Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode

  • Published:
Science in China Series B: Chemistry Aims and scope Submit manuscript

Abstract

An amperometric glucose biosensor was developed based on the immobilization of glucose oxidase in the organically modified silicate (ormosil)-polyvinyl acetate (PVA) matrix onto a Prussian Blue (PB)-modified glassy carbon electrode. A higher stability PB-modified electrode was prepared by the electrochemical deposition of FeCl3, K3[Fe(CN)6] and ethylenediamine tetraacetic acid (EDTA) under cyclic voltammetric (CV) conditions. The effects of the potential range of CV conditions, electrolyte cations, applied potential, pH, temperature and co-existing substances were investigated. The detection limit of the glucose biosensor was 8.1 μmol·L−1 (S/N = 3) with a linear range from 20 μmol·L−1 to 2 mmol·L−1 (R = 0.9965). The biosensor presented a fast response and good selectivity. Additionally, excellent reproducibility and stability of the biosensor were observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chen X H, Hu Y B, Wilson G S. Glucose microbiosensor based on alumina sol-gel matrix/electropolymerized composite membrane. Biosens Bioelectron, 2007, 17: 1005–1013

    Article  Google Scholar 

  2. Yang H, Chung T D, Kim Y T, Choi C A, Jun C H, Kim H C. Glucose sensor using a microfabricated electrode and electropolymerized bilayer films. Biosens Bioelectron, 2007, 17: 251–259

    Article  Google Scholar 

  3. Pandey P C, Upadhyay S. Bioelectrochemistry of glucose oxidase immobilized on ferrocene encapsulated ormosil modified electrode. Sens Actuators B Chem, 2001, 76: 193–198

    Article  Google Scholar 

  4. Karyakin A A, Karyakina E E, Gorton L. Prussian-blue-based amperotric biosensors in flow-injection analysis. Talanta, 1996, 43: 1597–1606

    Article  CAS  Google Scholar 

  5. Liu Z L, Liu H, Zhang M, Kong J L, Deng J Q. Al2O3 sol-gel derived amperometric biosensor for glucose. Anal Chim Acta, 1999, 392: 135–141

    Article  CAS  Google Scholar 

  6. Chi Q J, Dong S J. Amperometric biosensors based on the immobilization of oxidases in prussian blue film by electrochemical codeposition. Anal Chim Acta, 1995, 310: 429–436

    Article  CAS  Google Scholar 

  7. Li T, Ya Z H, Ding L. Development of an amperometric biosensor based on glucose oxidase immobilized through silica sol-gel film onto prussian blue modified electrode. Sens Actuators B Chem, 2004, 101: 155–160

    Article  CAS  Google Scholar 

  8. Tan X C, Tian Y X, Cai P X, Zou X Y. Glucose biosensor based on glucose oxidase immobilized in sol-gel chitosan/silica hybrid composite film on Prussian Blue modified glass carbon electrode. Anal Bioanal Chem, 2005, 381: 500–507

    Article  CAS  Google Scholar 

  9. Zhang N, Wilkop T, Lee S, Cheng Q. Bi-functionalization of a patterned prussian blue array for amperometric measurement of glucose via two integrated detection schemes. Analyst, 2007, 132: 164–172

    Article  CAS  Google Scholar 

  10. Xue M H, Xu Q, Zhou M, Zhu J J. In situ immobilization of glucose oxidase in chitosan-gold nanoparticle hybrid film on Prussian Blue modified electrode for high-sensitivity glucose detection. Electrochem Commun, 2006, 8: 1468–1474

    Article  CAS  Google Scholar 

  11. Xian Y Z, Hu Y, Liu F, Xian Y, Feng L J, Jin L T. Template synthesis of highly ordered prussian blue array and its application to the glucose biosensing. Biosens Bioelectron, 2007, 22: 2827–2833

    Article  CAS  Google Scholar 

  12. Yang C, Wang C H, Wu J S, Xia X H. Mechanism investigation of Prussian Blue electrochemically deposited from a solution containing single component of ferricyanide. Electrochim Acta, 2006, 51: 4019–4023

    Article  CAS  Google Scholar 

  13. Pan D W, Chen J H, Nie L H, Tao W Y, Yao S Z. Amperometric glucose biosensor based on immobilization of glucose oxidase in electropolymerized o-aminophenol film at Prussian Blue-modified platinum electrode. Electrochim Acta, 2004, 49: 795–801

    Article  CAS  Google Scholar 

  14. Pan D W, Chen J H, Nie L H, Tao W Y, Yao S Z. An amperometric glucose biosensor based on poly(o-aminophenol) and Prussian Blue films at platinum electrode. Anal Biochem, 2004, 324: 115–122

    Article  CAS  Google Scholar 

  15. Deng Q, Li B, Dong S J. Self-gelatinizable copolymer immobilized glucose biosensor based on Prussian Blue modified graphite electrode. Analyst, 1998, 123: 1995–1999

    Article  CAS  Google Scholar 

  16. Zhuang R F, Yang T Z. Preparation and behavior of higher stability Prussian Blue modified electrode. Inorg Chem, 1989, 5: 80–85

    CAS  Google Scholar 

  17. Brandy J, White H, Harmon J. Novel optical solid-state glucose sensor using immobilized glucose oxidase. Biochem Biophys Res Commun, 2002, 292: 1069–1071

    Google Scholar 

  18. Doretti L, Ferrara D, Gattolin P, Lora S. Covalently immobilized enzymes on biocompatible polymers for amperometric sensor applications. Biosens Bioelectron, 1996, 11: 365–373

    Article  CAS  Google Scholar 

  19. Li Z F, Kang E T, Neoh K G, Tan K L. Covalent immobilization of glucose oxidase on the surface of polyaniline films graft copolymerized with acrylic acid. Biomaterials, 1998, 19: 45–53

    Article  CAS  Google Scholar 

  20. Yang X H, Hua L, Gong H Q, Tan S N. Covalent immobilization of an enzyme (glucose oxidase) onto a carbon sol-gel silicate composite surface as a biosensing platform. Anal Chim Acta, 2003, 478: 67–75

    Article  CAS  Google Scholar 

  21. Chiu S H, Chung T W, Giridhar R, Wu W T. Immobilization of β-cyclodextrin in chitosan beads for separation of cholesterol from egg yolk. Food Res Int, 2004, 37: 217–223

    Article  CAS  Google Scholar 

  22. Yang Y H, Yang M H, Wang H, Tang L, Shen G L, Yu R Q. Inhibition biosensor for determination of nicotine. Anal Chim Acta, 2004, 509: 151–157

    Article  CAS  Google Scholar 

  23. Kwan R C H, Hon P Y T, Mak K K W, Renneberg R. Amperometric determination of lactate with novel trienzyme/poly(carbamoyl) sulfonate hydrogel-based sensor. Biosens Bioelectron, 2004, 19: 1745–1752

    Article  CAS  Google Scholar 

  24. Tag K, Lehmann M, Chan C Y, Renneberg R, Riedel K, Kunze G. Measurement of biodegradable substances with a myceliasensor based on the salt tolerant yeast Arxula adeninivorans LS3. Sens Actuators B Chem, 2000, 67: 142–148

    Article  Google Scholar 

  25. Ivanildo L M, Madalena C C A. Automated determination of glucose in soluble coffee using Prussian Blue-glucose oxidase-Nafion® modified electrode. Talanta, 2005, 66: 1281–1286

    Article  CAS  Google Scholar 

  26. Dodevska T, Horozova E, Dimcheva N. Electrocatalytic reduction of hydrogen peroxide on modified graphite electrodes: Application to the development of glucose biosensors. Anal Bioanal Chem, 2006, 386: 1413–1418

    Article  CAS  Google Scholar 

  27. Niculescu M, Gáspár S, Schulte A, Csöregi E, Schuhmann W. Visualization of micropatterned complex biosensor sensing chemistries by means of scanning electrochemical microscopy. Biosens Bioelectron, 2004, 19: 1175–1184

    Article  CAS  Google Scholar 

  28. Chen D D, Cao Y B, Liu B H, Kong J L. A BOD biosensor based on a microorganism immobilized on an Al2O3 sol-gel matrix. Anal Bioanal Chem, 2002, 372: 737–739

    Article  CAS  Google Scholar 

  29. Jiang Y Q, Xiao L L, Zhao L, Chen X, Wang X R, Wong K Y. Optical biosensor for the determination of BOD in seawater. Talanta, 2006, 70: 97–103

    Article  CAS  Google Scholar 

  30. Lin L, Xiao L L, Huang S, Zhao L, Cui J S, Wang X H, Chen X. Novel BOD optical fiber biosensor based on co-immobilized microorganisms in ormosils matrix. Biosens Bioelectron, 2006, 21: 1703–1709

    Article  CAS  Google Scholar 

  31. Nakane K, Yamashita T, Iwakora K, Suzuki F. Properties and structure of poly(vinyl alcohol)/silica composites. J Appl Polym Sci, 1999, 74: 133–138

    Article  CAS  Google Scholar 

  32. Zhang X J, Wang J, Ogorevc B, Spichiger U E. Glucose nanosensor based on Prussian-Blue modified carbon-fiber cone nanoelectrode and an integrated reference electrode. Electroanal, 1999, 11: 945–949

    Article  CAS  Google Scholar 

  33. Inzelt G. A quartz crystal microbalance study of the sorption of ions and solvent molecules in poly(tetracyanoquinodimethane) electrodes. J Electroanal Chem, 1990, 287: 171–177

    Article  CAS  Google Scholar 

  34. Bácskai J, Martinusz K, Czirók E, Inzelt G, Kulesza P J, Malik M A. Polynuclear nickel hexacyanoferrates: Monitoring of film growth and hydrated counter-cation flux/storage during redox reactions. J Electroanal Chem, 1995, 385: 241–248

    Article  Google Scholar 

  35. Herren F, Fischer P, Ludi A, Halg W. Neutron diffraction study of Prussian Blue, Fe4[Fe(CN)6]3·xH2O. Location of water molecules and long-range magnetic order. Inorg Chem, 1980, 19: 956–959

    CAS  Google Scholar 

  36. Buser H J, Sschwarzenbach D, Petter W, Ludi A. The crystal structure of Prussian Blue: Fe4[Fe(CN)6]3·xH2O. Inorg Chem, 1977, 16: 2704–2710

    Article  CAS  Google Scholar 

  37. Itaya K, Ataka T, Toshima S. Spectroelectrochemistry and electrochemical preparation method of Prussian Blue modified electrodes. J Am Chem Soc, 1982, 104: 4767–4772

    Article  CAS  Google Scholar 

  38. Dostal A, Kauschka G, Reddy S J, Scholz F. Lattice contractions and expansions accompanying the electrochemical conversions of Prussian Blue and the reversible and irreversible insertion of rubidium and thallium ions. J Electroanal Chem, 1996, 406: 155–163

    Article  Google Scholar 

  39. Malik M A, Miecznikowski K, Kulesza P J. Quartz crystal microbalance monitoring of mass transport during redox processes of cyanometallate modified electrodes: Complex charge transport in nickel hexacyanoferrate films. Electrochim Acta, 2000, 45: 3777–3784

    Article  CAS  Google Scholar 

  40. Abbaspour A, Kamyabi M A. Electrochemical formation of Prussian Blue films with a single ferricyanide solution on gold eletrode. J Electroanal Chem, 2005, 584: 117–123

    Article  CAS  Google Scholar 

  41. Pournaghi-Azar M H, Dastangoo H. Palladized aluminum as a novel substrate for the non-electrolytic preparation of a Prussian Blue film modified electrode. J Electroanal Chem, 2004, 573: 355–364

    Article  CAS  Google Scholar 

  42. Palma P, Paola B, Elisabetta A. PVA-gel (Lentikats) as an effective matrix for yeast strain immobilization aimed at heterologous protein production. Enzyme Microb Technol, 2006, 38: 184–189

    Article  CAS  Google Scholar 

  43. Karyakin A A. Prussian Blue and its analogues: Electrochemistry and analytical applications. Electroanal, 2001, 13: 813–819

    Article  CAS  Google Scholar 

  44. Yang X F, Zhou Z D, Xiao D, Choi M M F. A fluorescent glucose biosensor based on immobilized glucose oxidase on bamboo inner shell membrane. Biosens Bioelectron, 2006, 21: 1613–1620

    Article  CAS  Google Scholar 

  45. Wu B L, Zhang G M, Shuang S M, Choi M M F. Biosensors for determination of glucose with glucose oxidase immobilized on an eggshell membrane. Talanta, 2004, 64: 546–553

    Article  CAS  Google Scholar 

  46. Kamin R A, Wilson G S. Rotating ring-disk enzyme electrode for biocatalysis kinetic studies and characterization of immobilized enzyme layer. Anal Chem, 1980, 52: 1198–1205

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Testing Center for Food and Drug Safety, Huaxia Vocational College, Xiamen, 361005, China

    HaiLing Chen, Li Zhao, ZhiXia Zhuang & XiaoRu Wang

  2. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, China

    Xi Chen

Authors
  1. HaiLing Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xi Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. ZhiXia Zhuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. XiaoRu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xi Chen.

Additional information

Supported by the National High Technical Development Project (863 project) Foundation (Grant No. 2006AA09Z160) and the National Natural Science Foundation of China (Grant No. 20775064)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, H., Zhao, L., Chen, X. et al. Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase in an ormosil-PVA matrix onto a Prussian Blue modified electrode. Sci. China Ser. B-Chem. 52, 1128–1135 (2009). https://doi.org/10.1007/s11426-009-0146-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11426-009-0146-4

Keywords

Search

Navigation