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Molecular basis for antifreeze activity difference of two insect antifreeze protein isoforms

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Abstract

The insect spruce budworm (Choristoneura fumiferana) produces antifreeze protein (AFP) to assist in the protection of the over-wintering larval stage and contains multiple isoforms. Structures for two isoforms, known as CfAFP-501 and CfAFP-337, show that both possess similar left-handed β-helical structure, although thermal hysteresis activity of the longer isoform CfAFP-501 is three times that of CfAFP-337. The markedly enhanced activity of CfAFP-501 is not proportional to, and cannot be simply accounted for, by the increased ice-binding site resulting from the two extra coils in CfAFP-501. In order to investigate the molecular basis for the activity difference and gain better understanding of AFPs in general, we have employed several different computational methods to systematically study the structural properties and ice interactions of the AFPs and their deletion models. In the context of intact AFPs, a majority of the coils in CfAFP-501 has better ice interaction and causes stronger ice lattice disruption than CfAFP-337, strongly suggesting a cooperative or synergistic effect among β-helical coils. The synergistic effect would play a critical role and make significant contributions to the antifreeze activity β-helical antifreeze proteins. This is the first time that synergistic effect and its implication for antifreeze activity are reported for β-helical antifreeze proteins.

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Authors and Affiliations

  1. Department of Chemistry, Beijing Normal University, Beijing, 100875, China

    Zhou YanXia, Tan HongWei, Liu RuoZhuang & Chen GuangJu

  2. Faculty of Science, Beijing University of Chemical Technology, Beijing, 100029, China

    Yang ZuoYin

  3. Department of Biochemistry, Queen’s University, Kingston, Ontario, K7L3N6, Canada

    Jia ZongChao

Authors
  1. Zhou YanXia
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  2. Tan HongWei
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  3. Yang ZuoYin
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  4. Jia ZongChao
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  5. Liu RuoZhuang
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  6. Chen GuangJu
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Corresponding author

Correspondence to Chen GuangJu.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 26073011, 20231010, 20503002 and 20631020) and the Major State Basic Research Development Programs (Grant No. G2004CB719900)

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Zhou, Y., Tan, H., Yang, Z. et al. Molecular basis for antifreeze activity difference of two insect antifreeze protein isoforms. SCI CHINA SER B 50, 266–271 (2007). https://doi.org/10.1007/s11426-007-0027-7

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  • DOI: https://doi.org/10.1007/s11426-007-0027-7

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