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Electrostatic interaction mechanism on the separation of phenols by non-aqueous capillary electrophoresis

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Abstract

The electrostatic interaction between additive and analyte is of great importance to non-aqueous capillary electrophoresis (NACE) separation. Three tetraalkylammonium bromides and acetonitrile were applied as additives and running solvent respectively. The effect of alkyl chain length and concentration of additive on electrostatic interactions was investigated by the separation of phenols. The separation ability was found to increase with decreasing alkyl chain length of the additive, and the resolution values were increased with increasing additive concentration. The separation was seriously deteriorated after a little amount of water was added in the running solution. Furthermore, the electrostatic interaction is strong under the conditions of low electron cloud density, weak steric hindrance and multi-interaction sites. Thus, the separation result can be predicted by theoretical analysis, which is helpful for the separation of other substances in NACE based on electrostatic interaction.

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Authors and Affiliations

  1. Department of Pharmaceutics, College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China

    Wei WeiLi, Xia ZhiNing, Chen ZhiTao & Liu WeiQi

  2. Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing, 100081, China

    Yin YongGuang

  3. Key Laboratory for Biomechanics & Tissue Engineering of Ministry of Education, Chongqing University, Chongqing, 400044, China

    Xia ZhiNing & Chen ZhiTao

Authors
  1. Wei WeiLi
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  2. Yin YongGuang
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  3. Xia ZhiNing
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  4. Chen ZhiTao
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  5. Liu WeiQi
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Corresponding author

Correspondence to Xia ZhiNing.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 20375051) and the Excellent Young Teachers Program of Ministry of Education (Grant No. [2002]123)

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Wei, W., Yin, Y., Xia, Z. et al. Electrostatic interaction mechanism on the separation of phenols by non-aqueous capillary electrophoresis. SCI CHINA SER B 50, 47–53 (2007). https://doi.org/10.1007/s11426-007-0018-8

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  • DOI: https://doi.org/10.1007/s11426-007-0018-8

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