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In situ synthesis of DNA micro-arrays using typography technique

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Abstract

A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide, which has the celerity, high spatial resolution, lower cost, reliable operation, and high synthetic efficiency. The principle and process of the typography technique for fabricating gene-chips have been described in detail. A suit of poly(terafluoroethylene) devices for synthesizing oligonucleotide arrays were designed and prepared, and the fiber tubes with a number of nano-or micron-channels were employed. The oligonucleotide arrays of 16 and 160 features with four different probes were synthesized using the typography technique. The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals. It was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen single-nucleotide polymorphisms (SNP) and to detect mutations.

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China

    Tang JianXin, He NongYue & Li Song

  2. Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hunan University of Technology, Zhuzhou, 412008, China

    Tang JianXin & He NongYue

  3. Zhongshan Huoju Polytechnic, Zhongshan, 528436, China

    He NongYue

Authors
  1. Tang JianXin
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  2. He NongYue
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  3. Li Song
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Corresponding author

Correspondence to He NongYue.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 60571032, 60571001, 90606027 and 60121101), the National Hi-Tech Research and Development Program of China (Grant No. 2006AA03Z357), the Natural Science Foundation of Hunan Province (Grant Nos. 04jj40023 and 06JJ4012) and the Natural Science Foundation of Guangdong Province (Grant No. 04008782)

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Tang, J., He, N. & Li, S. In situ synthesis of DNA micro-arrays using typography technique. SCI CHINA SER B 50, 75–83 (2007). https://doi.org/10.1007/s11426-007-0005-0

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  • DOI: https://doi.org/10.1007/s11426-007-0005-0

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