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Enzymatic cyclization of linear peptide to plant cyclopeptide heterophyllin B

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Abstract

The crude enzyme (PH-1) isolated from Pseudostellaria heterophylla by our group has catalyzed enzymatic cyclization of linear peptide NH2-Gly1-Gly2-Leu-Pro-Pro-Pro-Ile-Phe-COOH (4) into cyclopeptide heterophyllin B (HB) from plant for the first time. To ensure this reaction, some analytical methods including TLC, HPLC, MS, NMR, and 3C labeling were used to prove that the reaction of substrate 4 sharing residue of NH-Phe-Gly-CO was successful.

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References

  1. Zhou, J., Tan, N. H., Application of a new TLC chemical method for detection of cyclopeptides in plants, Chinese Science Bulletin, 2000, 45(20): 1825–1831.

    CAS  Google Scholar 

  2. Kohli, R. M., Walsh, C. T., Burkart, M. D., Biomimetics synthesis and optimization of cyclic peptide antibiotics, Nature, 2002, 418(6898): 658–661.

    Article  CAS  Google Scholar 

  3. Kohli, R. M., Burke, M. D., Tao, J. H., Walsh, C. T., Chemoenzymatic route to macrocyclic hybrid peptide/polyketide-like molecules, J. Am. Chem. Soc., 2003, 125(24): 7160–7161.

    Article  CAS  Google Scholar 

  4. Jackson, D. Y., Burnier, J. P., Wells, J. A., Enzymatic cyclization of linear peptides esters using subtiligase, J. Am. Chem. Soc., 1995, 117(2): 819–820.

    Article  CAS  Google Scholar 

  5. Chinese Academy of Medical Sciences, Zhong Yao Zhi Vol. 2, Beijing: The People’s Health Publishing House, 1995, 266-268.

  6. Tan, N. H., Zhou, J., Chen, C. X., Zhao, S. X., Cyclopeptides from the root of Pseudostellaria heterophylla, Phytochemistry, 1993, 32(5): 1327–1330.

    CAS  Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Graduate University of Chinese Academy of Sciences, Chinese Academy of Sciences, Kunming, 650204, China

    Jia Aiqun, Tan Ninghua, Shen Yuemao & Zhou Jun

  2. Chinese Peptide Co., Ltd, Hangzhou, 310018, China

    Li Xiang

  3. Southwest Forestry College, 650204, Kunming, China

    Liu Xiaozhu

Authors
  1. Jia Aiqun
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  2. Li Xiang
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  3. Tan Ninghua
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  4. Liu Xiaozhu
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  5. Shen Yuemao
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  6. Zhou Jun
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Corresponding author

Correspondence to Zhou Jun.

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Jia, A., Li, X., Tan, N. et al. Enzymatic cyclization of linear peptide to plant cyclopeptide heterophyllin B. SCI CHINA SER B 49, 63–66 (2006). https://doi.org/10.1007/s11426-005-0204-5

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  • DOI: https://doi.org/10.1007/s11426-005-0204-5

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