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Journal of Systems Science and Complexity

, Volume 31, Issue 3, pp 581–595 | Cite as

Construction of Multilayer Porous Scaffold Based on Magnetically Guided Assembly of Microfiber

  • Xingfu Li
  • Huaping Wang
  • Qing Shi
  • Tao Sun
  • Qiang Huang
  • Toshio Fukuda
Article
  • 90 Downloads

Abstract

This paper proposes a novel method of magnetically guided assembly to construct multi-layer porous scaffold for three-dimensional cell culture by apply magnetic microfibers. Microfibers are composed of biocompatible and biodegradable alginate solution with homogeneous magnetic nanoparticles, which are continuously spun from a microfluidic device by precise pressure control of the syringe pump. Magnetic nanoparticles enable the control of magnetic field on microfibers. Meanwhile, magnetized device combining with a round permanent magnet are utilized to guide the distribution of spouted microfibers. The device is composed by pure iron wire arrays and wax, which stimulates powerful magnetic flux density and magnetic field gradients for the capture and assembly of microfibers. Thus, magnetic microfibers are spun on desired places of the magnetized device by motion control of the micromanipulation robot, and precise locations are adjusted by magnetic force couple with the assist of glass micropipette. Afterwards, microfibers are spatially organized by periodic magnetic force and crossed layer-by-layer to form micro-pore structure with both length and width of 650 μm. Finally, the authors construct a multilayer microfiber-based scaffold with high porosity to provide a satisfactory environment for long-term cell culture. The experimental results demonstrate the effectiveness of the proposed method.

Keywords

Magnetically guided assembly magnetic microfiber magnetized device periodic magnetic force multilayer porous scaffold 

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Copyright information

© Institute of Systems Science, Academy of Mathematics and Systems Science, CAS and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Xingfu Li
    • 1
    • 2
    • 3
  • Huaping Wang
    • 1
    • 2
    • 3
  • Qing Shi
    • 1
    • 2
    • 3
  • Tao Sun
    • 1
    • 2
    • 3
  • Qiang Huang
    • 1
    • 2
    • 3
  • Toshio Fukuda
    • 1
    • 2
    • 3
  1. 1.Intelligent Robotics Institute, School of Mechatronical EngineeringBeijing Institute of TechnologyBeijingChina
  2. 2.Key Laboratory of Biomimetic Robots and SystemsMinistry of EducationBeijingChina
  3. 3.Key Laboratory of Intelligent Control and Decision of Complex SystemsBeijingChina

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