Journal of Systems Science and Complexity

, Volume 31, Issue 3, pp 581–595 | Cite as

Construction of Multilayer Porous Scaffold Based on Magnetically Guided Assembly of Microfiber

  • Xingfu Li
  • Huaping Wang
  • Qing Shi
  • Tao Sun
  • Qiang Huang
  • Toshio Fukuda


This paper proposes a novel method of magnetically guided assembly to construct multi-layer porous scaffold for three-dimensional cell culture by apply magnetic microfibers. Microfibers are composed of biocompatible and biodegradable alginate solution with homogeneous magnetic nanoparticles, which are continuously spun from a microfluidic device by precise pressure control of the syringe pump. Magnetic nanoparticles enable the control of magnetic field on microfibers. Meanwhile, magnetized device combining with a round permanent magnet are utilized to guide the distribution of spouted microfibers. The device is composed by pure iron wire arrays and wax, which stimulates powerful magnetic flux density and magnetic field gradients for the capture and assembly of microfibers. Thus, magnetic microfibers are spun on desired places of the magnetized device by motion control of the micromanipulation robot, and precise locations are adjusted by magnetic force couple with the assist of glass micropipette. Afterwards, microfibers are spatially organized by periodic magnetic force and crossed layer-by-layer to form micro-pore structure with both length and width of 650 μm. Finally, the authors construct a multilayer microfiber-based scaffold with high porosity to provide a satisfactory environment for long-term cell culture. The experimental results demonstrate the effectiveness of the proposed method.


Magnetically guided assembly magnetic microfiber magnetized device periodic magnetic force multilayer porous scaffold 


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  1. [1]
    Badylak S F, A scaffold immune microenvironment, Science, 2016, 352(6283): 298–298.CrossRefGoogle Scholar
  2. [2]
    Ratner M, Shire punts on bioscaffolds for cell-based regenerative medicine, Nature Biotechnology, 2012, 30(8): 727–728.CrossRefGoogle Scholar
  3. [3]
    Zhang X J, Li Y, Chen Y E, et al., Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects, Nature Communications, 2016, 7: 10376–10390.CrossRefGoogle Scholar
  4. [4]
    Hollister S J, Porous scaffold design for tissue engineering, Nature Materials, 2005, 4(7): 518–524.CrossRefGoogle Scholar
  5. [5]
    Griffith L G and Naughton G, Tissue engineering-current challenges and expanding opportunities, Science, 2002, 295: 1009–1014.CrossRefGoogle Scholar
  6. [6]
    Matsunaga Y T, Morimoto Y, and Takeuchi S, Molding cell beads for rapid construction of macroscopic 3D tissue architecture, Advanced Materials, 2011, 23: H90–H94.CrossRefGoogle Scholar
  7. [7]
    Tamayol A, Akbari M, Annabi N, et al., Fiber-based tissue engineering: Progress, challenges and opportunities, Biotechnology Advances, 2013, 31(5): 669–687.CrossRefGoogle Scholar
  8. [8]
    Yue T, Nakajima M, Takeuchi M, et al., On-chip self-assembly of cell embedded microstructures to vascular-like microtubes, Lab on a Chip, 2014, 14(6): 1151–1161.CrossRefGoogle Scholar
  9. [9]
    Murphy S V and Anthony A, 3D bioprinting of tissue and organs, Nature Biotechnology, 2014, 32: 773–785.CrossRefGoogle Scholar
  10. [10]
    Durmus N G, Tasoglu S, and Demirci U, Bioprinting: Functional droplet networks, Nature Materials, 2013, 12(6): 478–479.CrossRefGoogle Scholar
  11. [11]
    Kang H W, Sang J L, Ko I K, et al., A 3D bioprinting system to produce human-scale tissue constructs with structural integrity, Nature Biotechnology, 2016, 34(3): 312–319.CrossRefGoogle Scholar
  12. [12]
    Lai K L and Xu T, Bioprinting of cartilage: Recent progress on bioprinting of cartilage, Nature, 2015, 88(20): 241–242.Google Scholar
  13. [13]
    Pawar A A, Saada G, Cooperstein I, et al., High-performance 3D printing of hydrogels by waterdispersible photoinitiator nanoparticles, Science Advances, 2016, 2(4): 1501381–1501387.CrossRefGoogle Scholar
  14. [14]
    Kolesky D B, Truby R L, Gladman A S, et al., 3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs, Advanced Materials, 2014, 26(19): 3124–3130.CrossRefGoogle Scholar
  15. [15]
    Zhao Y, Yao R, Ouyang L, et al., Three-dimensional printing of hela cells for cervical tumor model in vitro, Biofabrication, 2014, 6(3): 035001.CrossRefGoogle Scholar
  16. [16]
    Xu C X, Chai W X, Huang Y, et al., Scaffold-free inkjet printing of three-dimensional zigzag cellular tubes, NBiotechnology and Bioengineering, 2012, 109: 3152–3160.CrossRefGoogle Scholar
  17. [17]
    Duan B, Hockaday L A, Kang K H, et al., 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels, Journal of Biomedical Materials Research Part A, 2013, 101A: 1255–1264.Google Scholar
  18. [18]
    Wang H P, Huang Q, Shi Q, et al., Automated assembly of vascular-like microtube with repetitive single-step contact manipulation, IEEE Transactions on Biomedical Engineering, 2015, 62(11): 2620–2628.CrossRefGoogle Scholar
  19. [19]
    Wang H P, Shi Q, Guo Y N, et al., Contact assembly of cell-laden hollow microtubes through automated micromanipulator tip locating, Journal of Micromechanics and Microengineering, 2017, 27(1): 1–13.CrossRefGoogle Scholar
  20. [20]
    Zhang Y, Chen B K, Liu X Y, et al., Autonomous robotic pick-and-place of microobjects, IEEE Transactions on Robotics, 2010, 26: 200–207.CrossRefGoogle Scholar
  21. [21]
    Mol A, Van Lieshout M I, Dam-de Veen C G, et al., Fibrin as a cell carrier in cardiovascular tissue engineering applications, Biomaterials, 2005, 26(16): 3113–3121.CrossRefGoogle Scholar
  22. [22]
    Yu Y, Wen H, Ma J, et al., Flexible fabrication of biomimetic bamboo-like hybrid microfibers, Advanced Materials, 2014, 26(16): 2494–2499.CrossRefGoogle Scholar
  23. [23]
    Onoe H, Okitsu T, Itou A, et al., Metre-long cell-laden microfibers exhibit tissue morphologies and functions, Nature materials, 2013, 12: 584–590.CrossRefGoogle Scholar
  24. [24]
    Yamada M, Utoh R, Ohashi K, et al., Controlled formation of heterotypic hepatic micro-organoids in anisotropic hydrogel microfibers for long-term preservation of liver-specific functions, Biomaterials, 2012, 33(33): 8304–8315.CrossRefGoogle Scholar
  25. [25]
    Sun T, Hu C Z, Nakajima M, et al., On-chip fabrication and magnetic force estimation of peapodlike hybrid microfibers using a microfluidic device, Microfluidics and Nanofluidics, 2014, 18(5–6): 1177–1187.Google Scholar
  26. [26]
    Lee H W, Kim K C, and Lee J, Review of maglev train technologies, IEEE Transactions on Magnetics, 2006, 42(7): 1917–1925.CrossRefGoogle Scholar
  27. [27]
    Goya G F, Calatayud M P, Sanz B, et al., Magnetic nanoparticles for magnetically guided therapies against neural diseases, Mrs Bulletin, 2014, 39(11): 965–969.CrossRefGoogle Scholar
  28. [28]
    Tasoglu S, Diller E, Guven S, et al., Untethered micro-robotic coding of three-dimensional materials composition, Nature Communications, 2014, 5(1): 3124.CrossRefGoogle Scholar
  29. [29]
    Mejías R, Pérez-Yagüe S, Gutiérrez L, et al., Dimercaptosuccinic acid-coated magnetite nanoparticles for magnetically guided in vivo delivery of interferon gamma for cancer immunotherapy, Biomaterials, 2011, 32(11): 2938–2952.CrossRefGoogle Scholar
  30. [30]
    Fuchigami T, Kawamura R, Kitamoto Y, et al., A magnetically guided anti-cancer drug delivery system using porous FePt capsules, Biomaterials, 2012, 33(5): 1682–1687.CrossRefGoogle Scholar
  31. [31]
    Arcese L, Fruchard M, and Ferreira A, Endovascular magnetically guided robots: Navigation modeling and optimization, IEEE Transactions on Biomedical Engineering, 2012, 59(4): 977–987.CrossRefGoogle Scholar
  32. [32]
    Alsberg E, Feinstein E, Joy M P, et al., Magnetically-guided self-assembly of fibrin matrices with ordered nano-scale structure for tissue engineering, Tissue Engineering, 2006, 12(11): 3247–3256.CrossRefGoogle Scholar
  33. [33]
    Hu C Z, Uchida T, Tercero C, et al., Development of biodegradable scaffolds based on magnetically guided assembly of magnetic sugar particles, Journal of Biotechnology, 2012, 159(1–2): 90–98.CrossRefGoogle Scholar
  34. [34]
    He X H, Wang W, Liu Y M, et al., Microfluidic fabrication of bio-Inspired microfibers with controllable magnetic spindle-knots for 3D assembly and water collection, ACS Applied Materials & Interfaces, 2015, 7(31): 17471–17481.CrossRefGoogle Scholar

Copyright information

© Institute of Systems Science, Academy of Mathematics and Systems Science, CAS and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Xingfu Li
    • 1
    • 2
    • 3
  • Huaping Wang
    • 1
    • 2
    • 3
  • Qing Shi
    • 1
    • 2
    • 3
  • Tao Sun
    • 1
    • 2
    • 3
  • Qiang Huang
    • 1
    • 2
    • 3
  • Toshio Fukuda
    • 1
    • 2
    • 3
  1. 1.Intelligent Robotics Institute, School of Mechatronical EngineeringBeijing Institute of TechnologyBeijingChina
  2. 2.Key Laboratory of Biomimetic Robots and SystemsMinistry of EducationBeijingChina
  3. 3.Key Laboratory of Intelligent Control and Decision of Complex SystemsBeijingChina

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