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Quantification of clitidine in caps and stems of poisonous mushroom Paralepistopsis acromelalga by hydrophilic interaction liquid chromatography–tandem mass spectrometry

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Abstract

Purpose

A simple and high-throughput analytical method for determining clitidine in Paralepistopsis acromelalga using hydrophilic interaction liquid chromatography–tandem mass spectrometry (LC–MS/MS) was established.

Methods

To determine clitidine in the mushrooms, a simple procedure including dilution with methanol solution and filtering by cartridge was employed just before quantification by LC–MS/MS for high-throughput analysis. In this report, concentrations of clitidine in mushrooms were determined by the standard addition method.

Results

The present established method was successfully applied to the analysis of fruit bodies of P. acromelalga, which were obtained from five different locations in Japan. Results on concentrations of clitidine in each stem and cap of P. acromelalga specimens tested showed that their concentrations were quite different, not only between stems and caps, but also among locations and strains; the concentrations of clitidine in stems and caps ranged from 1.41 to 9.30 mg/g and 3.17 to 14.4 mg/g, respectively.

Conclusions

This is the first report to present a detailed quantitative analysis of clitidine by MS and the distribution of clitidine in stems and caps of P. acromelalga. This analytical method for clitidine was thought to be useful in P. acromelalga poisoning cases to identify the causative toxic mushroom.

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Acknowledgements

This work was supported by PhD development program of Inner Mongolia Medical University, grant number YKD2017BQ008. This work was also supported by research grant from Japan Society for the Promotion of Science KAKENHI Grant-in-Aid for Young Scientists (B), Grant number JP17K15880. This work was also supported by Health Labor Sciences Research Grant (H28-iyaku-ippan-006) of The Ministry of Health Labor and Welfare. A part of this work was also supported by Health Labor Sciences Research Grants (H27-shokuhin-ippan-007).

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Author notes

  1. Koutaro Hasegawa is also the co-first author for this article.

Authors and Affiliations

  1. Department of Legal Medicine, College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China

    Amin Wurita

  2. Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu 431-3192, Japan

    Koutaro Hasegawa, Kunio Gonmori, Kayoko Minakata, Hideki Nozawa, Itaru Yamagishi, Kanako Watanabe & Osamu Suzuki

  3. Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan

    Katsuhiro Konno

  4. Faculty of Life Sciences, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan

    Kimiko Hashimoto

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  1. Amin Wurita
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Corresponding author

Correspondence to Koutaro Hasegawa.

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Conflict of interest

The coauthor Osamu Suzuki is a Chief Editor of the Forensic Toxicology, but was not involved in the peer-reviewing of this article. Other authors declare no competing interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Wurita, A., Hasegawa, K., Konno, K. et al. Quantification of clitidine in caps and stems of poisonous mushroom Paralepistopsis acromelalga by hydrophilic interaction liquid chromatography–tandem mass spectrometry. Forensic Toxicol 37, 378–386 (2019). https://doi.org/10.1007/s11419-019-00470-5

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  • DOI: https://doi.org/10.1007/s11419-019-00470-5

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