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Simultaneous analysis of acephate and methamidophos in human serum by improved extraction and GC-MS

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Abstract

A detailed procedure for simple and rapid analysis of acephate, an organophosphorous pesticide, and its metabolite methamidophos in human serum by gas chromatography-mass spectrometry was established. The method included solid-phase extraction with activated charcoal, which gave high recoveries of both analytes. After validation of the method, it was successfully applied to a serum sample obtained from an actual poisoning case. The present method seems very useful, especially in forensic and environmental toxicology.

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Authors and Affiliations

  1. Department of Legal Medicine, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663-8501, Japan

    Nobuyuki Adachi, Hiroshi Kinoshita, Minori Nishiguchi, Motonori Takahashi, Harumi Ouchi, Takako Minami, Kiyoshi Matsui, Takehiko Yamamura, Nao Ohtsu, Shie Yoshida & Shigeru Hishida

  2. Joint-Use Research Facilities, Hyogo College of Medicine, Hyogo, Japan

    Nobuyuki Adachi

  3. Forensic Science Laboratory, Hyogo Prefectural Police Headquarters, Kobe, Japan

    Hiroyuki Motomura

Authors
  1. Nobuyuki Adachi
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  2. Hiroshi Kinoshita
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  3. Minori Nishiguchi
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  4. Motonori Takahashi
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  5. Harumi Ouchi
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  6. Takako Minami
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  7. Kiyoshi Matsui
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  8. Takehiko Yamamura
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  9. Hiroyuki Motomura
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  10. Nao Ohtsu
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  11. Shie Yoshida
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  12. Shigeru Hishida
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Corresponding author

Correspondence to Nobuyuki Adachi.

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Adachi, N., Kinoshita, H., Nishiguchi, M. et al. Simultaneous analysis of acephate and methamidophos in human serum by improved extraction and GC-MS. Forensic Toxicol 26, 76–79 (2008). https://doi.org/10.1007/s11419-008-0052-z

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  • DOI: https://doi.org/10.1007/s11419-008-0052-z

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