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New biflavonoids isolated from Xylia kerrii leaves extract with selective cytotoxicity against MH7A human rheumatoid arthritis synovial fibroblast cells

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Abstract

Three new biflavonoids (1–3) and two known flavonoids (4, 5) were isolated from Xylia kerrii collected in Thailand. Compounds 1–5 showed selective cytotoxicity against the rheumatoid fibroblast-like synovial MH7A cell line, and these compounds showed weak cytotoxicity against the human lung synovial fibroblast WI-38 VA13 sub 2 RA cell line. Notably, compound 1 was highly selective toward MH7A cells with an IC50 value of 6.9 μM, whereas the IC50 value for WI-38 VA13 sub 2 RA cells was > 100 μM. The western blotting analysis of MH7A cells treated with compound 1 showed increased CDKN2A /p16INK4A and caspase-8 levels.

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Acknowledgements

We thank the late Takashi Koyano (Temko Corporation) and late Thaworn Kowithayakorn (Khon Kaen University) for their collaboration in the collection and identification of plant materials.

Funding

This work was supported by KAKENHI (Grant nos. 20H03394, 20K16024, and 23K14369) from the Japan Society for the Promotion of Science, JST SPRING (Grant number JPMJSP2109), the Uehara Memorial Foundation, the Shorai Foundation for Science and Technology, and Kagawa University Research Promotion Program (Grant number 23K0F014).

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan

    Kazuki Fujii, Itsuki Iwata, Akiko Takaya, Masami Ishibashi & Yasumasa Hara

  2. Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8673, Japan

    Akiko Takaya

  3. School of Pharmacy at Fukuoka, International University of Health and Welfare, 137-1 Enokizu, Okawa, Fukuoka, 831-8501, Japan

    Masami Ishibashi

  4. Faculty of Agriculture, Kagawa University, 2393 Ikenobe, Miki, Kagawa, 761-0795, Japan

    Yasumasa Hara

Authors
  1. Kazuki Fujii
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  2. Itsuki Iwata
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  3. Akiko Takaya
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  4. Masami Ishibashi
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  5. Yasumasa Hara
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Contributions

Conceptualization, Y.H. and M.I.; isolation of compounds and biological experiment, K.F. and I.I.; data analysis, K.F., I.I., Y.H., A.T., and M.I.; writing—original draft preparation, K.F.; writing—review and editing, Y.H., A.T., and M.I. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Yasumasa Hara.

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Fujii, K., Iwata, I., Takaya, A. et al. New biflavonoids isolated from Xylia kerrii leaves extract with selective cytotoxicity against MH7A human rheumatoid arthritis synovial fibroblast cells. J Nat Med (2024). https://doi.org/10.1007/s11418-024-01801-6

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  • DOI: https://doi.org/10.1007/s11418-024-01801-6

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