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Investigation of microsatellite loci for the identification of registered varieties of Perilla frutescens and a discussion on the ancestor species of P. frutescens

  • Natural Resource Letter
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Abstract

Techniques for identifying varieties of crops used as spices and food additives have important implications for the safety of food production, prevention of false labeling, protection of breeders’ rights, and prevention of theft or outflow to other countries. Presently, there are 16 varieties of Perilla frutescens in the variety registration system of the Ministry of Agriculture, Forestry and Fishes in Japan (Ministry of Agriculture, Forestry and Fisheries. Variety registration data search. http://www.hinshu2.maff.go.jp/. Accessed 03 Nov 2022). One such variety is “Shimoadachi,” which contains citral as a main essential oil component and has a lemon-like smell. To our knowledge, no other cultivars with similar characteristics in P. frutescens have been identified. Additionally, the registered variety “per-001” contains high contents of perillaldehyde and rosmarinic acid, with practical applications for herbal medicines and functional foods. Therefore, the development of variety identification techniques is necessary for stable production and protection. In this study, we investigated microsatellite loci for the accurate identification of registered varieties of red perilla. These loci provide a basis for breeding superior varieties of medicinal plants.

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Data Availability Statement

The data that support the findings of this study are available from the corresponding author, Michiho Ito, upon reasonable request.

References

  1. Ministry of Agriculture, Forestry and Fisheries. Variety registration data search. http://www.hinshu2.maff.go.jp/. Accessed 03 Nov 2022

  2. Plant Variety Protection and Seed Act (Act No. 83 of May 29, 1998, Japan).

  3. Japan Seeds and Seedlings Association (2017). The basics of seeds and seedlings, illustrated and understandable, p. 110. Seibundo Shinkosya Publishing Co., Ltd. ISBN: 978-4-416-51791-8.

  4. Rural Culture Association (2020). Nobunkyo booklet 22-What do you think about Seed and Seedling Law for the future of seeds and seedlings?-. 19–20. Rural Culture Association. ISBN: 978-4-540-20174-5.

  5. Monden Y (2017) Development of crop cultivar discrimination method: for conserving valuable cultivars developed in Japan. Kagaku to Seibutsu 55(12):817–824. https://doi.org/10.1271/kagakutoseibutsu.55.817

    Article  CAS  Google Scholar 

  6. Yamada M, Besho H, Goto K (2016) The future of the fruit industry pioneered by new varieties and technologies. Agriculture and forestry statistics publishing Inc. ISBN: 978-4897323428

  7. The National Agriculture and Food Research Organization. Information on DNA variety identification technology using DNA. https://www.naro.go.jp/collab/breed/hinshu_shikibetsu/index.html. Accessed 03 11 2022

  8. Sa KJ, Lim SE, Choi I-Y, Kyong-Cheul Park Ju, Lee K (2019) Development and characterization of new microsatellite markers for Perilla frutescens (L.) Britton. Am J Plant Sci 10(9):1623–1630

    Article  CAS  Google Scholar 

  9. Lee PLM (2017) DNA amplification in the field: move over PCR, here comes LAMP. Mol Ecol Resour 17(2):138–141

    Article  CAS  PubMed  Google Scholar 

  10. Monden Y, Takasaki K, Futo S, Niwa K, Kawase M, Akitake H, Tahara M (2014) A rapid and enhanced DNA detection method for crop cultivar discrimination. J Biotechnol 185(20):57–62

    Article  CAS  PubMed  Google Scholar 

  11. Ito M, Honda G (2007) Geranniol synthases from perilla and their taxonomical significance. Phytochemistry 68(4):446–453

    Article  CAS  PubMed  Google Scholar 

  12. Ito M (1999) Studies on the taxonomy and chemotaxonomy of Japanese Perilla species. Kyoto University, Ph. D. thesis.

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Acknowledgements

We are grateful to Mae Chu Co., Ltd. and the National Institutes of Biomedical Innovation, Health and Nutrition for providing experimental materials. This research is supported by the Research on Regulatory Science of Pharmaceuticals and Medical Devices (22mk0101199), a project of Japan Agency for Medical Research and Development.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshidashimoadachi-cho, Sakyo-ku, Kyoto, 606-8501, Japan

    Yuya Deguchi & Michiho Ito

  2. Nagasaki Prefectural Institute for Environmental Research and Public Health, 2-1306-11 Ikeda, Omura, Nagasaki, 856-0026, Japan

    Yuya Deguchi

  3. Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, 210-9501, Japan

    Michiho Ito

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  1. Yuya Deguchi
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  2. Michiho Ito
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Correspondence to Michiho Ito.

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Deguchi, Y., Ito, M. Investigation of microsatellite loci for the identification of registered varieties of Perilla frutescens and a discussion on the ancestor species of P. frutescens. J Nat Med 77, 412–420 (2023). https://doi.org/10.1007/s11418-022-01676-5

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  • DOI: https://doi.org/10.1007/s11418-022-01676-5

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