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A new 1,2-diketone physalin isolated from Physalis minima and TRAIL-resistance overcoming activity of physalins

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Abstract

A new 1,2-diketone physalin, physalin XII (1), and 13 known compounds were isolated from the methanol extract of Physalis minima whole plant collected in Thailand. Among them, five physalins (2–6) had tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-resistance overcoming activity, and physalin F (3) was the most active with an IC50 value of 0.39 µM against human gastric adenocarcinoma cell line AGS in the presence of TRAIL (100 ng/mL). An investigation of the TRAIL-resistance overcoming activity of physalins using western blot analysis showed that 3 promoted TRAIL-induced apoptosis by suppressing anti-apoptotic proteins c-FLIP and Bcl-2.

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Data availability

The data that support the findings of this study are available from the corresponding authors upon reasonable request.

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Acknowledgements

We thank Dr. Takashi Koyano (Temko Corporation) and Prof. Thaworn Kowithayakorn (Khon Kaen University) for their collaboration in collecting and identifying plant materials. This work was supported by KAKENHI Grant Nos. 20H03394 and 20K16024 from the Japan Society for Promotion of Science.

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba, 260-8675, Japan

    Teruhisa Manome, Yasumasa Hara & Masami Ishibashi

  2. Plant Molecular Science Center, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba, 260-8675, Japan

    Yasumasa Hara & Masami Ishibashi

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  1. Teruhisa Manome
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  2. Yasumasa Hara
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Correspondence to Yasumasa Hara or Masami Ishibashi.

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Manome, T., Hara, Y. & Ishibashi, M. A new 1,2-diketone physalin isolated from Physalis minima and TRAIL-resistance overcoming activity of physalins. J Nat Med 77, 370–378 (2023). https://doi.org/10.1007/s11418-022-01670-x

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