Abstract
Many patients with allergies have anxiety about taking anti-allergic medicines due to their side effects and increased medical expenses. Thus, developing functional foods/agricultural products for allergy prevention is strongly desired. In this study, we revealed that a Citrus flavanone, hesperetin, amplified IgE/antigen-mediated degranulation-inhibitory potency of anti-allergic catechin, (–)-epigallocatechin-3-O-(3-O-methyl) gallate (EGCG3ʹʹMe), in the rat basophilic/mast cell line RBL-2H3. Hesperetin also significantly elevated the activation of acid sphingomyelinase (ASM), essential for eliciting anti-allergic effect of EGCG3ʹʹMe through the cell surficial protein, 67-kDa laminin receptor (67LR). Furthermore, oral administration of the highly absorbent hesperidin, α-glucosyl hesperidin, also enhanced the inhibitory potency of EGCG3ʹʹMe-rich ‘Benifuuki’ green tea (Camellia sinensis L.) on passive cutaneous anaphylaxis (PCA) reaction evoked by IgE/antigen in BALB/c mice. These observations indicate that hesperetin amplifies the ability of EGCG3ʹʹMe to inhibit the IgE/antigen-mediated degranulation through activating ASM signaling.
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Data Availability
The data that support the findings of this study are available from the corresponding author on reasonable request.
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Acknowledgements
We would like to thank Editage (www.editage.com) for English language editing. This work was financially supported by JSPS KAKENHI [grant Numbers JP20H05683 (to H.T.) and JP20H02935 (to Y.F.)].
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Fujimura, Y., Yoshimoto, T., Fujino, K. et al. Bioactivity-boosting strategy based on combination of anti-allergic O-methylated catechin with a Citrus flavanone, hesperetin. J Nat Med 77, 363–369 (2023). https://doi.org/10.1007/s11418-022-01668-5
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DOI: https://doi.org/10.1007/s11418-022-01668-5