Abstract
Aplydactylonins A-C (1–3), three new sesquiterpenes, were isolated from the Vietnamese sea hare Aplysia dactylomela. Their structures and absolute configurations were elucidated based on spectroscopic analysis, X-ray crystallography, and density functional theory (DFT) calculations of NMR and ECD data. Compound 2 exhibited cytotoxicity against HepG2, DU145 and A549 cells with respective IC50 values of 4.08 ± 0.63, 38.64 ± 1.04 and 12.33 ± 0.95 µM. In addition, HepG2 cells treated with 5 μM compound 2 for 48 h showed a significant increase in early apoptotic cells (P < 0.05) and increased caspase 3 activity (P < 0.01). Moreover, compound 2 induced sub-G1 phase arrest in HepG2 cells.
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Acknowledgements
This work was supported by the Vietnam Academy of Science and Technology (Grant number: KHCBBI.02/19-21). We thank Dr. Dang Vu Luong (Institute of Chemistry, VAST, Hanoi, Vietnam) for NMR spectra measurement.
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Huong, P.T.M., Phong, N.V., Huong, N.T. et al. Aplydactylonins A-C, three new sesquiterpenes from the Vietnamese sea hare Aplysia dactylomela and their cytotoxicity. J Nat Med 76, 210–219 (2022). https://doi.org/10.1007/s11418-021-01582-2
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DOI: https://doi.org/10.1007/s11418-021-01582-2