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Identification of Valeriana fauriei and other Eurasian medicinal valerian by DNA polymorphisms in psbA–trnH intergenic spacer sequences in chloroplast DNA

Abstract

In order to differentiate among Valeriana fauriei Briq. and other Eurasian medicinal valerian (V. dioica L., V. hardwickii Wall., V. jatamansi Jones, and V. officinalis L.), we attempted to establish DNA markers. DNA sequences for the psbA–trnH intergenic spacer region of chloroplast DNA (psbA–trnH) and 18S ribosomal RNA, internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA, internal transcribed spacer 2 (ITS2), and 28S ribosomal RNA of nuclear DNA in V. fauriei and other Eurasian medicinal valerian were compared. Using partial sequences of psbA–trnH (nucleotide positions 1–75 from the 5′ end of the intergenic spacer region), V. fauriei and other Eurasian medicinal valerian could be correctly identified to the species level. In addition, the partial sequences of psbA–trnH in V. fauriei contained five different haplotypes, and it was possible to distinguish the origins of valerian from Japan and Eurasia (China and Korea). On the other hand, individuals had heterogeneous sequences of ITS1 and ITS2, making it impossible to use direct sequencing and DNA markers of ITS1 and ITS2 to distinguish species and origins of V. fauriei and other Eurasian medicinal valerian.

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References

  1. Hong D, Barrie FR, Bell CD (2011) Valeriana Linnaeus. In: Flora of China 19. Science Press, Beijing, pp 666–671

  2. Mathela CS, Chanotiya CS, Sammal SS, Pant AK, Pandey S (2005) Compositional diversity of terpenoids in the Himalayan Valeriana genera. Chem Biodivers 2:1174–1182

    CAS  Article  Google Scholar 

  3. Yamazaki T (2003) Valeriana L. In: Iwatsuki K, Yamazaki T, Boufford DE, Ohba H (eds) Flora of Japan, vol IIIa. Kodansha, Tokyo, pp 452–453

    Google Scholar 

  4. Ministry of Health, Labour and Welfare (2016) Japanese Valerian. In: The Japanese Pharmacopoeia, Seventeenth edn. Ministry of Health, Labour and Welfare, Tokyo, pp 1884–1885

  5. Yoshitomi S, Watanabe M, Kawanishi F, Satake M (2000) Quality and their sedative effects of Japanese Valerian roots. Nat Med 54:55–60 (in Japanese with English summary)

    CAS  Google Scholar 

  6. Yanagisawa K (2016) Transition of psychotropic drugs in Japanese Pharmacopoeia (JP) (Part 18) The study for new knowledge and decisions regarding cultivation method for Japanese Valerian (Kisso). Jpn J History Pharm 51:96–103 (in Japanese with English summary)

    Google Scholar 

  7. Yamamoto Y, Ko H, Sasaki H, Takeda O, Higuchi Y, Mukaida Y, Mori Y, Yamaguchi Y, Shiratori M (2019) Survey on crude drug usage in Japan. Shoyakugaku Zasshi 73:16–35 (in Japanese with English summary)

    Google Scholar 

  8. Kim CM, Ryu KS (1976) Studies on the constituents of Korean Valerians (I) Screening tests on the volatile oils from Korean Valeriana fauriei species. Kor J Pharmacog 7:237–240 (in Korean with English summary)

    Google Scholar 

  9. Kim B, Ma SS, Jo C, Lee S, Choi H, Lee K, Ham I, Choi HY (2019) Vasorelaxant effect of the ethanol extract from Valeriana fauriei Briquet root and rhizome on rat thoracic aorta. Pharmacogn Mag 15:59–65

    CAS  Article  Google Scholar 

  10. Zhao Z (2017) Study on chemical constituents of Valeriana fauriei Briq. in Jilin Province. Open J Nat Sci 5:99–105 (in Chinese with English summary)

    Article  Google Scholar 

  11. Manandhar NP (2002) Valeriana hardwickii Wallich; Valeriana jatamansii Jones. In: Plants and people of Nepal. Timber Press, Oregon, p 471

  12. Kapoor LD (2001) Valeriana jatamansi Jones. In: Handbook of Ayurvedic medicinal plants. CRC Press, Boca Raton, p 330

  13. Fernández S, Wasowski C, Paladini AC, Marder M (2004) Sedative and sleep-enhancing properties of linarin, a flavonoid-isolated from Valeriana officinalis. Pharmacol Biochem Behav 77:399–404

    Article  Google Scholar 

  14. Takeuchi M, Suzuki S, Fujino H, Arisawa M, Kohda H (2001) Evaluation of Valeriana plants and their improvement for crude drug production 1. Phylogenetic relationship in the genus Valeriana as revealed by RAPD markers. Nat Med 55:17–22

    CAS  Google Scholar 

  15. Bhagyawant SS (2016) RAPD-SCAR markers: an interface tool for authentication of traits. J Biosci Med 4:1–9

    CAS  Google Scholar 

  16. Suzuki H, Zhang BC, Harada M, Iida O, Satake M (1993) Quantitative studies on terpenes of Japanese and European valerians. Shoyakugaku Zasshi 47:305–310

    CAS  Google Scholar 

  17. Grassi F, Imazio S, Gomarasca S, Citterio S, Aina R, Sgorbati S, Sala F, Patrignani G, Labra M (2004) Population structure and genetic variation within Valeriana wallrothii Kreyer in relation to different ecological locations. Plant Sci 166:1437–1441

    CAS  Article  Google Scholar 

  18. Bell CD, Donoghue MJ (2005) Phylogeny and biogeography of Valerianaceae (Dipsacales) with special reference to the South American valerians. Org Divers Evol 5:147–159

    Article  Google Scholar 

  19. Yang Y, Zhai Y, Liu T, Zhang F, Ji Y (2011) Detection of Valeriana jatamansi as an adulterant of medicinal Paris by length variation of chloroplast psbA–trnH region. Planta Med 77:87–91

    CAS  Article  Google Scholar 

  20. Schmiderer C, Lukas B, Novak J (2013) Effect of different DNA extraction methods and DNA dilutions on the amplification success in the PCR of different medical and aromatic plants. J Med Spice Plants 18:65–72

    CAS  Google Scholar 

  21. Sang T, Crawford DJ, Stuessy TF (1997) Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Am J Bot 84:1120–1136

    CAS  Article  Google Scholar 

  22. Long C, Kakiuchi N, Takahashi A, Komatsu K, Cai S, Mikage M (2004) Phylogenetic analysis of the DNA sequence of the non-coding region of nuclear ribosomal DNA and chloroplast of Ephedra plants in China. Planta Med 70:1080–1084

    CAS  Article  Google Scholar 

  23. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    CAS  Article  Google Scholar 

  24. CBOL Plant Working Group (2009) A DNA barcode for land plants. PNAS 106:12794–12797

    Article  Google Scholar 

  25. Vijayan K, Tsou CH (2010) DNA barcoding in plants: taxonomy in a new perspective. Curr Sci 99:1530–1541

    CAS  Google Scholar 

  26. Techen N, Parveen I, Pan Z, Khan IA (2014) DNA barcoding of medicinal plant material for identification. COBIOT 25:103–110

    CAS  Google Scholar 

  27. Hennell JR, D’Agostino PM, Lee S, Khoo CS, Sucher NJ (2012) Using GeneBank®for genomic authentication: a tutorial. In: Sucher N, Hennell J, Carles M (eds) Plant DNA fingerprinting and barcoding: methods and protocols, methods in molecular biology, vol 862. Humana Press, New York, pp 181–200

    Chapter  Google Scholar 

  28. Bidartondo MI (2008) Preserving accuracy in GenBank. Science 319:1616

    CAS  Article  Google Scholar 

  29. Tanabe AS, Toju H (2013) Two new computational methods for universal DNA barcoding: a benchmark using barcode sequences of bacteria, archaea, animals, fungi, and land plants. PLoS ONE 11(3):e0152242

    Article  Google Scholar 

  30. Tsubota H, Inoue Y, Nakahara-Tsubota M, Mukai S (2014) DNA Barcoding as a supporting tool for identification of undetermined herbarium specimens, with special reference for dried plant specimens. Bullet Hiroshima University Museum 6:41–49 (in Japanese with English summary)

    Google Scholar 

  31. Takeuchi M, Suzuki S, Fujino H, Arisawa M, Kohda H (2001) Evaluation of Valeriana plants and their improvement for crude drug production 3. F1 hybrids production and their sesquiterpenoids contents. Nat Med 55:231–234

    CAS  Google Scholar 

  32. Takeuchi M, Suzuki Y, Suzuki S, Fujino H, Yoshizaki M, Kohda H (2001) Evaluation of Valeriana plants and their improvement for crude drug production 2. Diversity of sesquiterpenoid contents. Nat Med 55:225–230

    CAS  Google Scholar 

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Correspondence to Taichi Fujii.

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Fujii, T., Mori, T., Tatsuo, Y. et al. Identification of Valeriana fauriei and other Eurasian medicinal valerian by DNA polymorphisms in psbA–trnH intergenic spacer sequences in chloroplast DNA. J Nat Med 75, 699–706 (2021). https://doi.org/10.1007/s11418-021-01488-z

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  • DOI: https://doi.org/10.1007/s11418-021-01488-z

Keywords

  • Valeriana fauriei
  • Eurasian medicinal valerian
  • psbA–trnH intergenic spacer
  • ITS